Determination of functional activity of human iPSC-derived hepatocytes by measurement of CYP Metabolism

Pooja Chaudhari, Neha Prasad, Lipeng Tian, Yoon Young Jang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The advent of induced pluripotent stem cell (iPSC) technology has enabled the modeling of an array of specific human disease phenotypes, aiding in the increasingly important and indispensable understanding of disease progression and pathogenesis. Pluripotent stem cell-derived hepatocytes present a new avenue for drug screening and personalized drug testing toward precision medicine. CYP450 microsomal enzymes play a critical role in drug metabolism. Hence, CYP activity measurement of iPSC-derived hepatocytes is a vital prerequisite, to ensure metabolic functionality before proceeding to drug testing. Herein, we describe the protocol for measurement of different CYP450 enzyme activities in human iPSC-derived hepatocytes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages383-394
Number of pages12
Volume1357
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1357
ISSN (Print)10643745

Fingerprint

Induced Pluripotent Stem Cells
Hepatocytes
Pharmaceutical Preparations
Pluripotent Stem Cells
Precision Medicine
Preclinical Drug Evaluations
Enzymes
Disease Progression
Technology
Phenotype

Keywords

  • CYP450
  • Drug metabolism
  • Hepatic differentiation
  • iPSCs
  • Liver function

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Chaudhari, P., Prasad, N., Tian, L., & Jang, Y. Y. (2016). Determination of functional activity of human iPSC-derived hepatocytes by measurement of CYP Metabolism. In Methods in Molecular Biology (Vol. 1357, pp. 383-394). (Methods in Molecular Biology; Vol. 1357). Humana Press Inc.. https://doi.org/10.1007/7651_2014_145

Determination of functional activity of human iPSC-derived hepatocytes by measurement of CYP Metabolism. / Chaudhari, Pooja; Prasad, Neha; Tian, Lipeng; Jang, Yoon Young.

Methods in Molecular Biology. Vol. 1357 Humana Press Inc., 2016. p. 383-394 (Methods in Molecular Biology; Vol. 1357).

Research output: Chapter in Book/Report/Conference proceedingChapter

Chaudhari, P, Prasad, N, Tian, L & Jang, YY 2016, Determination of functional activity of human iPSC-derived hepatocytes by measurement of CYP Metabolism. in Methods in Molecular Biology. vol. 1357, Methods in Molecular Biology, vol. 1357, Humana Press Inc., pp. 383-394. https://doi.org/10.1007/7651_2014_145
Chaudhari P, Prasad N, Tian L, Jang YY. Determination of functional activity of human iPSC-derived hepatocytes by measurement of CYP Metabolism. In Methods in Molecular Biology. Vol. 1357. Humana Press Inc. 2016. p. 383-394. (Methods in Molecular Biology). https://doi.org/10.1007/7651_2014_145
Chaudhari, Pooja ; Prasad, Neha ; Tian, Lipeng ; Jang, Yoon Young. / Determination of functional activity of human iPSC-derived hepatocytes by measurement of CYP Metabolism. Methods in Molecular Biology. Vol. 1357 Humana Press Inc., 2016. pp. 383-394 (Methods in Molecular Biology).
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