Determination of ethylenediamineplatinum(II) malonate in infusion fluids, human plasma and urine by high-performance liquid chromatography

W. J F Van Der Vijgh, F. Elferink, G. J. Postma, J. B. Vermorken, H. M. Pinedo

Research output: Contribution to journalArticlepeer-review

Abstract

A selective and convenient high-performance liquid chromatographic assay was developed for ethylenediamineplatinum(II) malonate (JM-40) in plasma ultrafiltrate and urine. A μPorasil silica column (30 cm) was used with acetonitrile-water (90:10, v/v) as the mobile phase and the elution of compounds was monitored by ultraviolet absorbance at 214 nm. A linear dynamic range of at least three decades (1-1000 μg/ml) was achieved. The detection limit in plasma ultrafiltrate was 0.35 μg/ml. The stability of JM-40 was determined in 0.9% sodium chloride, 5% glucose, plasma ultrafiltrate and urine. More stable drug solutions were obtained with 5% glucose than with 0.9% sodium chloride. JM-40 was also determined in plasma ultrafiltrate and urine samples of one patient receiving short-term infusions of the drug. In plasma ultrafiltrate unmetabolized JM-40 was detected during the first 5 h after administration and had a half-life of 21.3 ± 1.6 min. The parent drug was excreted in the urine in rapidly decreasing amounts. Eighteen per cent of the dose was recovered as unmetabolized drug during the first 6 h.

Original languageEnglish (US)
Pages (from-to)335-342
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume310
Issue numberC
DOIs
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

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