Determination of circulating 25,26-dihydroxycholecalciferol in man by radioimmunoassay

L. J. Fraher, Thomas Clemens, S. E. Papapoulos, J. Redel, J. L. O'Riordan

Research output: Contribution to journalArticle

Abstract

A radioimmunoassay for 25,26-dihydroxycholecalciferol has been developed with an antiserum raised in a sheep, tritiated 1,25-dihydroxycholecalciferol as tracer and synthetic 25,26-dihydroxycholecalciferol as standard. The metabolite was purified from serum extracts by Sephadex LH 20 and high-pressure liquid chromatography; recovery was monitored with biologically generated, tritiated 25,26-dihydroxycholecalciferol. The mean ± SEM concentration of 25,26-dihydroxycholecalciferol in serum from 18 healthy subjects was 587 ± 65 pmol/l. Seven Asian patients with osteomalacia due to vitamin D deficiency had very low or undetectable (

Original languageEnglish (US)
Pages (from-to)257-263
Number of pages7
JournalClinical Science
Volume59
Issue number4
StatePublished - 1980
Externally publishedYes

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Radioimmunoassay
Osteomalacia
Vitamin D Deficiency
Calcitriol
Serum
Immune Sera
Sheep
Healthy Volunteers
High Pressure Liquid Chromatography
25,26-dihydroxycholecalciferol

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Fraher, L. J., Clemens, T., Papapoulos, S. E., Redel, J., & O'Riordan, J. L. (1980). Determination of circulating 25,26-dihydroxycholecalciferol in man by radioimmunoassay. Clinical Science, 59(4), 257-263.

Determination of circulating 25,26-dihydroxycholecalciferol in man by radioimmunoassay. / Fraher, L. J.; Clemens, Thomas; Papapoulos, S. E.; Redel, J.; O'Riordan, J. L.

In: Clinical Science, Vol. 59, No. 4, 1980, p. 257-263.

Research output: Contribution to journalArticle

Fraher, LJ, Clemens, T, Papapoulos, SE, Redel, J & O'Riordan, JL 1980, 'Determination of circulating 25,26-dihydroxycholecalciferol in man by radioimmunoassay', Clinical Science, vol. 59, no. 4, pp. 257-263.
Fraher, L. J. ; Clemens, Thomas ; Papapoulos, S. E. ; Redel, J. ; O'Riordan, J. L. / Determination of circulating 25,26-dihydroxycholecalciferol in man by radioimmunoassay. In: Clinical Science. 1980 ; Vol. 59, No. 4. pp. 257-263.
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