Determination of cerebral glucose transport and metabolic kinetics by dynamic MR spectroscopy

P. C.M. Van Zijl, D. Davis, S. M. Eleff, C. T.W. Moonen, R. J. Parker, J. M. Strong

Research output: Contribution to journalArticlepeer-review


A new in vivo nuclear magnetic resonance (NMR) spectroscopy method is introduced that dynamically measures cerebral utilization of magnetically labeled [1-13C]glucose from the change in total brain glucose signals on infusion. Kinetic equations are derived using a four-compartment model incorporating glucose transport and phosphorylation. Brain extract data show that the glucose 6-phosphate concentration is negligible relative to glucose, simplifying the kinetics to three compartments and allowing direct determination of the glucose-utilization half-life time [t( 1/4 ) = 1n2/(k2 + k3)] from the time dependence of the NMR signal. Results on isofluorane (n = 5)- and halothane (n = 7)-anesthetized cats give a hyperglycemic t( 1/4 ) = 5.10 ± 0.11 min-1 (SE). Using Michaelis-Menten kinetics and an assumed half- saturation constant K(t) = 5 ± 1 mM, we determined a maximal transport rate T(max) = 0.83 ± 0.19 μmol · g-1 · min-1, a cerebral metabolic rate of glucose CMR(Glc) = 0.22 ± 0.03 μmol · g-1 · min-1, and a normoglycemic cerebral influx rate CIR(Glc) = 0.37 ± 0.05 μmol · g-1 · min-1. Possible extension of this approach to positron emission tomography and proton NMR is discussed.

Original languageEnglish (US)
Pages (from-to)E1216-E1227
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number6 36-6
StatePublished - 1997


  • Brain
  • Cat
  • Michaelis-Menten kinetics
  • Nuclear magnetic resonance spectroscopy
  • [C]glucose utilization

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)


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