Determination of catecholamine permeability coefficients for passive diffusion across phospholipid vesicle membranes

Amy Bochain; Lorrie Estey; Grace Haronian; Michael Reale; Camilo Rojas; John Cramer

doi:10.1007/BF01870834

Determination of catecholamine permeability coefficients for passive diffusion across phospholipid vesicle membranes

Amy Bochain, Lorrie Estey, Grace Haronian, Michael Reale, Camilo Rojas, John Cramer

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A convenient catecholamine transport assay has been developed which permits continuous, instantaneous monitoring of transmembrane flux. Epinephrine transport has been examined by spectrophotometrically monitoring adrenochrome formation resulting from the passive diffusion of catecholamine into unilamellar phospholipid vesicles containing entrapped potassium ferricyanide. Ferricyanide oxidation of epinephrine under the conditions employed is fast compared to membrane transport, which obviates the need for intravesicular concentration or volume determinations. Epinephrine transport data over a pH 6 to 7 range have been fitted to an integrated rate equation from which a permeability coefficient for neutral epinephrine of 2.7±1.5×10^-6 cm/sec has been obtained.

Original language	English (US)
Pages (from-to)	73-76
Number of pages	4
Journal	The Journal of Membrane Biology
Volume	60
Issue number	1
DOIs	https://doi.org/10.1007/BF01870834
State	Published - Feb 1 1981
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Physiology
Cell Biology

Access to Document

10.1007/BF01870834

Cite this

@article{e1f254e310df4967b80e02e54a430510,

title = "Determination of catecholamine permeability coefficients for passive diffusion across phospholipid vesicle membranes",

abstract = "A convenient catecholamine transport assay has been developed which permits continuous, instantaneous monitoring of transmembrane flux. Epinephrine transport has been examined by spectrophotometrically monitoring adrenochrome formation resulting from the passive diffusion of catecholamine into unilamellar phospholipid vesicles containing entrapped potassium ferricyanide. Ferricyanide oxidation of epinephrine under the conditions employed is fast compared to membrane transport, which obviates the need for intravesicular concentration or volume determinations. Epinephrine transport data over a pH 6 to 7 range have been fitted to an integrated rate equation from which a permeability coefficient for neutral epinephrine of 2.7±1.5×10-6 cm/sec has been obtained.",

author = "Amy Bochain and Lorrie Estey and Grace Haronian and Michael Reale and Camilo Rojas and John Cramer",

year = "1981",

month = feb,

day = "1",

doi = "10.1007/BF01870834",

language = "English (US)",

volume = "60",

pages = "73--76",

journal = "The Journal of Membrane Biology",

issn = "0022-2631",

publisher = "Springer New York",

number = "1",

}

TY - JOUR

T1 - Determination of catecholamine permeability coefficients for passive diffusion across phospholipid vesicle membranes

AU - Bochain, Amy

AU - Estey, Lorrie

AU - Haronian, Grace

AU - Reale, Michael

AU - Rojas, Camilo

AU - Cramer, John

PY - 1981/2/1

Y1 - 1981/2/1

N2 - A convenient catecholamine transport assay has been developed which permits continuous, instantaneous monitoring of transmembrane flux. Epinephrine transport has been examined by spectrophotometrically monitoring adrenochrome formation resulting from the passive diffusion of catecholamine into unilamellar phospholipid vesicles containing entrapped potassium ferricyanide. Ferricyanide oxidation of epinephrine under the conditions employed is fast compared to membrane transport, which obviates the need for intravesicular concentration or volume determinations. Epinephrine transport data over a pH 6 to 7 range have been fitted to an integrated rate equation from which a permeability coefficient for neutral epinephrine of 2.7±1.5×10-6 cm/sec has been obtained.

AB - A convenient catecholamine transport assay has been developed which permits continuous, instantaneous monitoring of transmembrane flux. Epinephrine transport has been examined by spectrophotometrically monitoring adrenochrome formation resulting from the passive diffusion of catecholamine into unilamellar phospholipid vesicles containing entrapped potassium ferricyanide. Ferricyanide oxidation of epinephrine under the conditions employed is fast compared to membrane transport, which obviates the need for intravesicular concentration or volume determinations. Epinephrine transport data over a pH 6 to 7 range have been fitted to an integrated rate equation from which a permeability coefficient for neutral epinephrine of 2.7±1.5×10-6 cm/sec has been obtained.

UR - http://www.scopus.com/inward/record.url?scp=0019479267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019479267&partnerID=8YFLogxK

U2 - 10.1007/BF01870834

DO - 10.1007/BF01870834

M3 - Article

C2 - 7241583

AN - SCOPUS:0019479267

SN - 0022-2631

VL - 60

SP - 73

EP - 76

JO - The Journal of Membrane Biology

JF - The Journal of Membrane Biology

IS - 1

ER -

Determination of catecholamine permeability coefficients for passive diffusion across phospholipid vesicle membranes

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this