Determination of catecholamine permeability coefficients for passive diffusion across phospholipid vesicle membranes

Amy Bochain, Lorrie Estey, Grace Haronian, Michael Reale, Camilo Rojas, John Cramer

Research output: Contribution to journalArticle

Abstract

A convenient catecholamine transport assay has been developed which permits continuous, instantaneous monitoring of transmembrane flux. Epinephrine transport has been examined by spectrophotometrically monitoring adrenochrome formation resulting from the passive diffusion of catecholamine into unilamellar phospholipid vesicles containing entrapped potassium ferricyanide. Ferricyanide oxidation of epinephrine under the conditions employed is fast compared to membrane transport, which obviates the need for intravesicular concentration or volume determinations. Epinephrine transport data over a pH 6 to 7 range have been fitted to an integrated rate equation from which a permeability coefficient for neutral epinephrine of 2.7±1.5×10-6 cm/sec has been obtained.

Original languageEnglish (US)
Pages (from-to)73-76
Number of pages4
JournalThe Journal of Membrane Biology
Volume60
Issue number1
DOIs
StatePublished - Feb 1 1981
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

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