TY - JOUR
T1 - Determination of busulfan in human plasma by gas chromatography with electron-capture detection
AU - Chen, Tian Ling
AU - Grochow, Louise B.
AU - Hurowitz, Lisa A.
AU - Brundrett, Robert B.
N1 - Funding Information:
This work was supported in part by Grant 5 PO1 CA15396-15 from National Cancer Institute, National Institutes of Health. We are grateful to Ms. Ann Lar-occa for typing this manuscript.
PY - 1988
Y1 - 1988
N2 - A simple and highly sensitive gas chromatographic method has been developed for the determination of busulfan in human plasma. After extraction of plasma specimens (clinical or spiked) with ethyl acetate, busulfan and the internal standard [1,8-bis (methanesulfonyloxy) octane] were derivatized with 2,3,5,6-tetrafluorothiophenol to yield compounds monitored by a 63Ni electron-capture detector. Sample recoveries from extraction and derivatization were greater than 78 and 91%, respectively. The limit of quantitation was 0.01 μg/ml (0.04μM) in 1 ml of plasma with a linear relationship over the 0.01-1.0μg/ml (0.04-4μM) concentration range. The method has been applied to analyze the plasma versus time profile of busulfan in human subjects following administration of an oral dose of 4 mg/kg per day as a marrow ablative chemotherapy for bone marrow transplantation.
AB - A simple and highly sensitive gas chromatographic method has been developed for the determination of busulfan in human plasma. After extraction of plasma specimens (clinical or spiked) with ethyl acetate, busulfan and the internal standard [1,8-bis (methanesulfonyloxy) octane] were derivatized with 2,3,5,6-tetrafluorothiophenol to yield compounds monitored by a 63Ni electron-capture detector. Sample recoveries from extraction and derivatization were greater than 78 and 91%, respectively. The limit of quantitation was 0.01 μg/ml (0.04μM) in 1 ml of plasma with a linear relationship over the 0.01-1.0μg/ml (0.04-4μM) concentration range. The method has been applied to analyze the plasma versus time profile of busulfan in human subjects following administration of an oral dose of 4 mg/kg per day as a marrow ablative chemotherapy for bone marrow transplantation.
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U2 - 10.1016/0378-4347(88)80034-3
DO - 10.1016/0378-4347(88)80034-3
M3 - Article
C2 - 3372644
AN - SCOPUS:0023901651
SN - 0378-4347
VL - 425
SP - 303
EP - 309
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - C
ER -