Determination of busulfan in human plasma by gas chromatography with electron-capture detection

Tian Ling Chen, Louise B. Grochow, Lisa A. Hurowitz, Robert B. Brundrett

Research output: Contribution to journalArticlepeer-review

Abstract

A simple and highly sensitive gas chromatographic method has been developed for the determination of busulfan in human plasma. After extraction of plasma specimens (clinical or spiked) with ethyl acetate, busulfan and the internal standard [1,8-bis (methanesulfonyloxy) octane] were derivatized with 2,3,5,6-tetrafluorothiophenol to yield compounds monitored by a 63Ni electron-capture detector. Sample recoveries from extraction and derivatization were greater than 78 and 91%, respectively. The limit of quantitation was 0.01 μg/ml (0.04μM) in 1 ml of plasma with a linear relationship over the 0.01-1.0μg/ml (0.04-4μM) concentration range. The method has been applied to analyze the plasma versus time profile of busulfan in human subjects following administration of an oral dose of 4 mg/kg per day as a marrow ablative chemotherapy for bone marrow transplantation.

Original languageEnglish (US)
Pages (from-to)303-309
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume425
Issue numberC
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Chemistry(all)

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