Determination of air-borne pyrogens by the in vitro pyrogen test (IPT) based on human whole blood cytokine response

I. Kindinger, S. Fennrich, B. Zucker, G. Linsel, Th Hartung

Research output: Contribution to journalReview articlepeer-review

Abstract

The impact of environmental germs as well as their fragments and components, especially endotoxins, on human health is increasingly recognized. Different syndromes were described in connection with inhaled air-borne microbiological contamination, e.g. sick-building-syndrome, humidifier lung, organic dust toxic syndrome (OPTS) and "Monday-illness". Air-conditioning systems intensify this problem, as well as collection of biological waste in households, which represents a substantial source of air-borne pollution. In 1995, we described a new method for the detection of pyrogenic (fever-inducing), contaminations. This whole blood test (in vitro pyrogen test: IPT) uses the natural reaction of the immune system to detect very sensitively a broad spectrum of pyrogens in the relevant species. Injectable drugs represent the main application. Here, the test was adapted for the detection of air-borne pyrogens. Endotoxins, Gram-positive pyrogens, fungal spores as well as live bacteria were detected. The test offers the advantage that filters can be incubated directly after air sampling without any extraction procedures. The detection limit was 0.3 endotoxin units. In animal stables, up to 3 × 10 6 endotoxin equivalent units were found per cubic meter of air.

Original languageEnglish (US)
Pages (from-to)499-507+568
JournalVDI Berichte
Issue number1656
StatePublished - Dec 1 2002
Externally publishedYes

ASJC Scopus subject areas

  • Engineering(all)

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