Determination of a viral load threshold to distinguish symptomatic versus asymptomatic rotavirus infection in a high-disease-Burden African population

A. Bennett, N. Bar-Zeev, K. C. Jere, J. E. Tate, U. D. Parashar, O. Nakagomi, R. S. Heyderman, N. French, M. Iturriza-Gomara, N. A. Cunliffe

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshold cycle value (26.7) that predicts rotavirus-attributable gastroenteritis in this population. These data will inform the evaluation of direct and indirect rotavirus vaccine effects in Africa.

Original languageEnglish (US)
Pages (from-to)1951-1954
Number of pages4
JournalJournal of clinical microbiology
Volume53
Issue number6
DOIs
StatePublished - Jun 1 2015
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

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