Determination of 15-keto-13,14-dihydro-metabolites of PGE2 and PGF in plasma using high performance liquid chromatography and gas chromatography-mass spectrometry

W. C. Hubbard, J. T. Watson

Research output: Contribution to journalArticlepeer-review

Abstract

A method is described for the measurement of 15-keto-13,14-dihydrometabolites of PGE2 and PGF in peripheral human plasma. This involves purification by high performance liquid chromatography followed by determination of levels by combined gas chromatography-mass spectrometry using tetradeuterated analogs of the metabolites as internal standards. The levels of these metabolites in plasma are considered to be a more reasonable index of the entry of PGE2 and PGF into peripheral blood than are the levels of the corresponding primary prostaglandins. The endogenous levels of 15-keto-13,14-dihydro-PGE2 and 15-keto-13,14-dihydro-PGF found in peripheral plasma are 33 ± 10 pg/ml (SD; n=6) and 40 ± 16 pg/ml (SD; n=6), respectively.

Original languageEnglish (US)
Pages (from-to)21-35
Number of pages15
JournalProstaglandins
Volume12
Issue number1
DOIs
StatePublished - Jul 1976

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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