Determination of 15-keto-13,14-dihydro-metabolites of PGE2 and PGF2α in plasma using high performance liquid chromatography and gas chromatography-mass spectrometry

W. C. Hubbard, J. T. Watson

Research output: Contribution to journalArticle

Abstract

A method is described for the measurement of 15-keto-13,14-dihydrometabolites of PGE2 and PGF in peripheral human plasma. This involves purification by high performance liquid chromatography followed by determination of levels by combined gas chromatography-mass spectrometry using tetradeuterated analogs of the metabolites as internal standards. The levels of these metabolites in plasma are considered to be a more reasonable index of the entry of PGE2 and PGF into peripheral blood than are the levels of the corresponding primary prostaglandins. The endogenous levels of 15-keto-13,14-dihydro-PGE2 and 15-keto-13,14-dihydro-PGF found in peripheral plasma are 33 ± 10 pg/ml (SD; n=6) and 40 ± 16 pg/ml (SD; n=6), respectively.

Original languageEnglish (US)
Pages (from-to)21-35
Number of pages15
JournalProstaglandins
Volume12
Issue number1
DOIs
StatePublished - 1976
Externally publishedYes

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Dinoprost
High performance liquid chromatography
Metabolites
Dinoprostone
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
High Pressure Liquid Chromatography
Plasmas
Plasma (human)
Prostaglandins
Purification
Blood
15-keto-13,14-dihydroprostaglandin E2

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Determination of 15-keto-13,14-dihydro-metabolites of PGE2 and PGF2α in plasma using high performance liquid chromatography and gas chromatography-mass spectrometry. / Hubbard, W. C.; Watson, J. T.

In: Prostaglandins, Vol. 12, No. 1, 1976, p. 21-35.

Research output: Contribution to journalArticle

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