Determination of 15-keto-13,14-dihydro-metabolites of PGE2 and PGF2α in plasma using high performance liquid chromatography and gas chromatography-mass spectrometry

W. C. Hubbard; J. T. Watson

doi:10.1016/S0090-6980(76)80004-4

Determination of 15-keto-13,14-dihydro-metabolites of PGE₂ and PGF_2α in plasma using high performance liquid chromatography and gas chromatography-mass spectrometry

W. C. Hubbard, J. T. Watson

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

A method is described for the measurement of 15-keto-13,14-dihydrometabolites of PGE₂ and PGF_2α in peripheral human plasma. This involves purification by high performance liquid chromatography followed by determination of levels by combined gas chromatography-mass spectrometry using tetradeuterated analogs of the metabolites as internal standards. The levels of these metabolites in plasma are considered to be a more reasonable index of the entry of PGE₂ and PGF_2α into peripheral blood than are the levels of the corresponding primary prostaglandins. The endogenous levels of 15-keto-13,14-dihydro-PGE₂ and 15-keto-13,14-dihydro-PGF_2α found in peripheral plasma are 33 ± 10 pg/ml (SD; n=6) and 40 ± 16 pg/ml (SD; n=6), respectively.

Original language	English (US)
Pages (from-to)	21-35
Number of pages	15
Journal	Prostaglandins
Volume	12
Issue number	1
DOIs	https://doi.org/10.1016/S0090-6980(76)80004-4
State	Published - Jul 1976
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Endocrinology

Access to Document

10.1016/S0090-6980(76)80004-4

Cite this

@article{cde473ebd1cc491590fdfaef0f48d89d,

title = "Determination of 15-keto-13,14-dihydro-metabolites of PGE2 and PGF2α in plasma using high performance liquid chromatography and gas chromatography-mass spectrometry",

abstract = "A method is described for the measurement of 15-keto-13,14-dihydrometabolites of PGE2 and PGF2α in peripheral human plasma. This involves purification by high performance liquid chromatography followed by determination of levels by combined gas chromatography-mass spectrometry using tetradeuterated analogs of the metabolites as internal standards. The levels of these metabolites in plasma are considered to be a more reasonable index of the entry of PGE2 and PGF2α into peripheral blood than are the levels of the corresponding primary prostaglandins. The endogenous levels of 15-keto-13,14-dihydro-PGE2 and 15-keto-13,14-dihydro-PGF2α found in peripheral plasma are 33 ± 10 pg/ml (SD; n=6) and 40 ± 16 pg/ml (SD; n=6), respectively.",

author = "Hubbard, {W. C.} and Watson, {J. T.}",

note = "Funding Information: This work was supported by NIH Grant GM 15431-09. JTW was supported by NIH Grant GM 50290-04. We are grateful to: a) Dr. B. J. Sweetman for his advice in the development of the GC-MS assays and in the preparation of the manuscript; b) R. K. Carr for his advice in the development of the HPLC program and c) Mrs. Gudrun Moustafa for her technical assistance in processing the samples. The tetradeuterated analogs of 15K-H2-E 2 and 15K-H2-F2e were generous gifts of the Upjohn Co. (Drs. John E. Pike and Udo Axen).",

year = "1976",

month = jul,

doi = "10.1016/S0090-6980(76)80004-4",

language = "English (US)",

volume = "12",

pages = "21--35",

journal = "Prostaglandins",

issn = "0090-6980",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - Determination of 15-keto-13,14-dihydro-metabolites of PGE2 and PGF2α in plasma using high performance liquid chromatography and gas chromatography-mass spectrometry

AU - Hubbard, W. C.

AU - Watson, J. T.

N1 - Funding Information: This work was supported by NIH Grant GM 15431-09. JTW was supported by NIH Grant GM 50290-04. We are grateful to: a) Dr. B. J. Sweetman for his advice in the development of the GC-MS assays and in the preparation of the manuscript; b) R. K. Carr for his advice in the development of the HPLC program and c) Mrs. Gudrun Moustafa for her technical assistance in processing the samples. The tetradeuterated analogs of 15K-H2-E 2 and 15K-H2-F2e were generous gifts of the Upjohn Co. (Drs. John E. Pike and Udo Axen).

PY - 1976/7

Y1 - 1976/7

N2 - A method is described for the measurement of 15-keto-13,14-dihydrometabolites of PGE2 and PGF2α in peripheral human plasma. This involves purification by high performance liquid chromatography followed by determination of levels by combined gas chromatography-mass spectrometry using tetradeuterated analogs of the metabolites as internal standards. The levels of these metabolites in plasma are considered to be a more reasonable index of the entry of PGE2 and PGF2α into peripheral blood than are the levels of the corresponding primary prostaglandins. The endogenous levels of 15-keto-13,14-dihydro-PGE2 and 15-keto-13,14-dihydro-PGF2α found in peripheral plasma are 33 ± 10 pg/ml (SD; n=6) and 40 ± 16 pg/ml (SD; n=6), respectively.

AB - A method is described for the measurement of 15-keto-13,14-dihydrometabolites of PGE2 and PGF2α in peripheral human plasma. This involves purification by high performance liquid chromatography followed by determination of levels by combined gas chromatography-mass spectrometry using tetradeuterated analogs of the metabolites as internal standards. The levels of these metabolites in plasma are considered to be a more reasonable index of the entry of PGE2 and PGF2α into peripheral blood than are the levels of the corresponding primary prostaglandins. The endogenous levels of 15-keto-13,14-dihydro-PGE2 and 15-keto-13,14-dihydro-PGF2α found in peripheral plasma are 33 ± 10 pg/ml (SD; n=6) and 40 ± 16 pg/ml (SD; n=6), respectively.

UR - http://www.scopus.com/inward/record.url?scp=0017111650&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017111650&partnerID=8YFLogxK

U2 - 10.1016/S0090-6980(76)80004-4

DO - 10.1016/S0090-6980(76)80004-4

M3 - Article

C2 - 959575

AN - SCOPUS:0017111650

SN - 0090-6980

VL - 12

SP - 21

EP - 35

JO - Prostaglandins

JF - Prostaglandins

IS - 1

ER -

Determination of 15-keto-13,14-dihydro-metabolites of PGE₂ and PGF_2α in plasma using high performance liquid chromatography and gas chromatography-mass spectrometry

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this