A rapid, sensitive and selective determination of δ9-tetrahydrocannabinol (THC)_in human plasma, serum and saliva was developed with high-performance liquid chromatography with electrochemical detection. Initially, samples were deproteinized, followed by a one step liquid-liquid extraction. Samples were measured by high-performance liquid chromatography with electrochemical detection with 4-dodecylresorcinol as the internal standard. The minimal detectable limit for THC in biological samples was ca. 1 ng/ml with a signal-to-noise ratio >3, corresponding to an on-column sensitivity for THC of ca. 0.5 ng. The detector was operated at +0.90 V vs. Ag/AgCl and exhibited linearity over a concentration range of 1-150 ng/ml with correlation coefficients of the standard curves > 0.99.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|State||Published - 1987|
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