TY - JOUR
T1 - Determination of δ9-tetrahydrocannabinol in human blood and saliva by high-performance liquid chromatography with amperometric detection
AU - Thompson, Loren K.
AU - Cone, Edward J.
PY - 1987
Y1 - 1987
N2 - A rapid, sensitive and selective determination of δ9-tetrahydrocannabinol (THC)_in human plasma, serum and saliva was developed with high-performance liquid chromatography with electrochemical detection. Initially, samples were deproteinized, followed by a one step liquid-liquid extraction. Samples were measured by high-performance liquid chromatography with electrochemical detection with 4-dodecylresorcinol as the internal standard. The minimal detectable limit for THC in biological samples was ca. 1 ng/ml with a signal-to-noise ratio >3, corresponding to an on-column sensitivity for THC of ca. 0.5 ng. The detector was operated at +0.90 V vs. Ag/AgCl and exhibited linearity over a concentration range of 1-150 ng/ml with correlation coefficients of the standard curves > 0.99.
AB - A rapid, sensitive and selective determination of δ9-tetrahydrocannabinol (THC)_in human plasma, serum and saliva was developed with high-performance liquid chromatography with electrochemical detection. Initially, samples were deproteinized, followed by a one step liquid-liquid extraction. Samples were measured by high-performance liquid chromatography with electrochemical detection with 4-dodecylresorcinol as the internal standard. The minimal detectable limit for THC in biological samples was ca. 1 ng/ml with a signal-to-noise ratio >3, corresponding to an on-column sensitivity for THC of ca. 0.5 ng. The detector was operated at +0.90 V vs. Ag/AgCl and exhibited linearity over a concentration range of 1-150 ng/ml with correlation coefficients of the standard curves > 0.99.
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U2 - 10.1016/0378-4347(87)80382-1
DO - 10.1016/0378-4347(87)80382-1
M3 - Article
C2 - 2828395
AN - SCOPUS:0023203573
SN - 0378-4347
VL - 421
SP - 91
EP - 97
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - C
ER -