Determination of δ9-tetrahydrocannabinol in human blood and saliva by high-performance liquid chromatography with amperometric detection

Loren K. Thompson, Edward J. Cone

Research output: Contribution to journalArticle

Abstract

A rapid, sensitive and selective determination of δ9-tetrahydrocannabinol (THC)_in human plasma, serum and saliva was developed with high-performance liquid chromatography with electrochemical detection. Initially, samples were deproteinized, followed by a one step liquid-liquid extraction. Samples were measured by high-performance liquid chromatography with electrochemical detection with 4-dodecylresorcinol as the internal standard. The minimal detectable limit for THC in biological samples was ca. 1 ng/ml with a signal-to-noise ratio >3, corresponding to an on-column sensitivity for THC of ca. 0.5 ng. The detector was operated at +0.90 V vs. Ag/AgCl and exhibited linearity over a concentration range of 1-150 ng/ml with correlation coefficients of the standard curves > 0.99.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume421
Issue numberC
DOIs
StatePublished - 1987

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Dronabinol
High performance liquid chromatography
Saliva
Blood
High Pressure Liquid Chromatography
Plasma (human)
Liquid-Liquid Extraction
Liquids
Signal-To-Noise Ratio
Signal to noise ratio
Detectors
Serum

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

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title = "Determination of δ9-tetrahydrocannabinol in human blood and saliva by high-performance liquid chromatography with amperometric detection",
abstract = "A rapid, sensitive and selective determination of δ9-tetrahydrocannabinol (THC)_in human plasma, serum and saliva was developed with high-performance liquid chromatography with electrochemical detection. Initially, samples were deproteinized, followed by a one step liquid-liquid extraction. Samples were measured by high-performance liquid chromatography with electrochemical detection with 4-dodecylresorcinol as the internal standard. The minimal detectable limit for THC in biological samples was ca. 1 ng/ml with a signal-to-noise ratio >3, corresponding to an on-column sensitivity for THC of ca. 0.5 ng. The detector was operated at +0.90 V vs. Ag/AgCl and exhibited linearity over a concentration range of 1-150 ng/ml with correlation coefficients of the standard curves > 0.99.",
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AU - Cone, Edward J.

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AB - A rapid, sensitive and selective determination of δ9-tetrahydrocannabinol (THC)_in human plasma, serum and saliva was developed with high-performance liquid chromatography with electrochemical detection. Initially, samples were deproteinized, followed by a one step liquid-liquid extraction. Samples were measured by high-performance liquid chromatography with electrochemical detection with 4-dodecylresorcinol as the internal standard. The minimal detectable limit for THC in biological samples was ca. 1 ng/ml with a signal-to-noise ratio >3, corresponding to an on-column sensitivity for THC of ca. 0.5 ng. The detector was operated at +0.90 V vs. Ag/AgCl and exhibited linearity over a concentration range of 1-150 ng/ml with correlation coefficients of the standard curves > 0.99.

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