Determination by DNA hybridization of Shiga-like-toxin-producing Escherichia coli in children with diarrhea in Thailand

J. E. Brown, P. Echeverria, D. N. Taylor, J. Seriwatana, V. Vanapruks, U. Lexomboon, R. N. Neill, J. W. Newland

Research output: Contribution to journalArticle

Abstract

Specific DNA probes were used to identify Shiga-like-toxin (SLT) I- and II-producing Escherichia coli from children less than 5 years of age with bloody diarrhea, in infants with diarrhea, and in controls of the same age without diarrhea in Thailand. At one hospital, SLT-producing E. coli was identified in 4 (7%) of 54 children with bloody diarrhea from whom other enteric pathogens were not identified and from 3 (6%) of 50 children without diarrhea. In the positive specimens, SLT-producing E. coli constituted only 0.3 to 4% of the 100 to 300 colonies on the replica blots. Non-toxin-encoding 933J and 933W bacteriophagelike DNA sequence were detected by colony hybridization with E. coli isolates from 18 (33%) of 54 children whith bloody diarrhea and 23 (46%) of 50 controls. At another hospital, SLT-producing E. coli was not identified in 115 infants with diarrhea and 119 controls without diarrhea. One infant with diarrhea was infected with E. coli O76:H7 that hybridized with the enterohemorrhagic E. coli probe but not with the SLT probes. E. coli producing SLT I or SLT II was isolated in small numbers from a similar proportion of Thai children with bloody diarrhea in whom no other enteric pathogen was identified and from controls without diarrhea.

Original languageEnglish (US)
Pages (from-to)291-294
Number of pages4
JournalJournal of clinical microbiology
Volume27
Issue number2
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Determination by DNA hybridization of Shiga-like-toxin-producing Escherichia coli in children with diarrhea in Thailand'. Together they form a unique fingerprint.

  • Cite this

    Brown, J. E., Echeverria, P., Taylor, D. N., Seriwatana, J., Vanapruks, V., Lexomboon, U., Neill, R. N., & Newland, J. W. (1989). Determination by DNA hybridization of Shiga-like-toxin-producing Escherichia coli in children with diarrhea in Thailand. Journal of clinical microbiology, 27(2), 291-294.