Determinants of endocytic membrane geometry, stability, and scission

Takuma Kishimoto, Yidi Sun, Christopher Buser, Jian Liu, Alphée Michelot, David G. Drubin

Research output: Contribution to journalArticle

Abstract

During endocytic vesicle formation, distinct subdomains along the membrane invagination are specified by different proteins, which bend the membrane and drive scission. Bin-Amphiphysin-Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR) proteins can induce membrane curvature and have been suggested to facilitate membrane invagination and scission. Two F-BAR proteins, Syp1 and Bzz1, are found at budding yeast endocytic sites. Syp1 arrives early but departs from the endocytic site before formation of deep membrane invaginations and scission. Using genetic, spatiotemporal, and ultrastructural analyses, we demonstrate that Bzz1, the heterodimeric BAR domain protein Rvs161/167, actin polymerization, and the lipid phosphatase Sjl2 cooperate, each through a distinct mechanism, to induce membrane scission in yeast. Additionally, actin assembly and Rvs161/167 cooperate to drive formation of deep invaginations. Finally, we find that Bzz1, acting at the invagination base, stabilizes endocytic sites and functions with Rvs161/167, localized along the tubule, to achieve proper endocytic membrane geometry necessary for efficient scission. Together, our results reveal that dynamic interplay between a lipid phosphatase, actin assembly, and membrane-sculpting proteins leads to proper membrane shaping, tubule stabilization, and scission.

Original languageEnglish (US)
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number44
DOIs
StatePublished - Nov 1 2011
Externally publishedYes

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Membranes
Actins
Phosphoric Monoester Hydrolases
Spatio-Temporal Analysis
Lipids
Transport Vesicles
Proteins
Saccharomycetales
Polymerization
Membrane Proteins
Yeasts
amphiphysin

Keywords

  • Cytoskeleton
  • Electron microscopy
  • Endocytosis

ASJC Scopus subject areas

  • General

Cite this

Determinants of endocytic membrane geometry, stability, and scission. / Kishimoto, Takuma; Sun, Yidi; Buser, Christopher; Liu, Jian; Michelot, Alphée; Drubin, David G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 44, 01.11.2011.

Research output: Contribution to journalArticle

Kishimoto, Takuma ; Sun, Yidi ; Buser, Christopher ; Liu, Jian ; Michelot, Alphée ; Drubin, David G. / Determinants of endocytic membrane geometry, stability, and scission. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 44.
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