Abstract
This protocol describes how to identify Dual Expressers (DEs), a rare type of lymphocyte that co-expresses B-cell receptors and T-cell receptors, by flow cytometry using a cocktail of four antibodies. It also shows the subsequent gating strategy for detecting and sorting DEs and the generation of EBV-immortalized DE lymphoblastoid cell lines and clones for antibody production and cloning antigen receptors. Use of this protocol maximizes detection of DEs and minimizes inclusion of doublets. For complete details on the use and execution of this protocol, please refer to Ahmed et al. (2019).
Original language | English (US) |
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Article number | 100925 |
Journal | STAR Protocols |
Volume | 2 |
Issue number | 4 |
DOIs | |
State | Published - Dec 17 2021 |
Keywords
- Antibody
- Cell Biology
- Cell culture
- Cell isolation
- Flow Cytometry/Mass Cytometry
- Immunology
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- General Neuroscience
- General Immunology and Microbiology
- General Medicine