TY - JOUR
T1 - Detection of two biological markers of intercourse
T2 - Prostate-specific antigen and Y-chromosomal DNA
AU - Jamshidi, Roxanne
AU - Penman-Aguilar, Ana
AU - Wiener, Jeffrey
AU - Gallo, Maria F.
AU - Zenilman, Jonathan M.
AU - Melendez, J. H.
AU - Snead, Margaret
AU - Black, Carolyn M.
AU - Jamieson, Denise J.
AU - Macaluso, Maurizio
N1 - Funding Information:
This publication/project was made possible through a Cooperative Agreement between the CDC and the Association of Teachers of Preventive Medicine (ATPM) , award number TS-1327 . The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the CDC or ATPM. The authors express gratitude to Dr. Marcia Hobbs for her valuable scientific review of the manuscript.
PY - 2013/12
Y1 - 2013/12
N2 - Background Although biological markers of women's exposure to semen from vaginal intercourse have been developed as surrogates for risk of infection or probability of pregnancy, data on their persistence time and clearance are limited. Study Design During 2006-2008, 52 couples were enrolled for three 14-day cycles of abstinence from vaginal sex during which women were exposed in the clinic to a specific quantity (10, 100 or 1000 μL) of their partner's semen. Vaginal swabs were collected before and at 1, 6, 12, 24, 48, 72 and 144 h after exposure for testing for prostate-specific antigen (PSA) and Y-chromosome DNA (Yc DNA). Results Immediately after exposure to 1000 μL of semen, the predicted sensitivity of being PSA positive was 0.96; this decreased to 0.65, 0.44, 0.21 and 0.07 at 6, 12, 24 and 48 h, respectively. Corresponding predicted sensitivity of being Yc DNA positive was 0.72 immediately postexposure; this increased to 0.76 at 1 h postexposure and then decreased to 0.60 (at 6 h), 0.63 (at 12 h), 0.49 (at 24 h), 0.21 (at 48 h), 0.17 (at 72 h) and 0.12 (at 144 h). Conclusions Overall findings suggest that PSA may be more consistent as a marker of very recent exposure and that Yc DNA is more likely to be detected in the vagina after 12 h postexposure compared to PSA.
AB - Background Although biological markers of women's exposure to semen from vaginal intercourse have been developed as surrogates for risk of infection or probability of pregnancy, data on their persistence time and clearance are limited. Study Design During 2006-2008, 52 couples were enrolled for three 14-day cycles of abstinence from vaginal sex during which women were exposed in the clinic to a specific quantity (10, 100 or 1000 μL) of their partner's semen. Vaginal swabs were collected before and at 1, 6, 12, 24, 48, 72 and 144 h after exposure for testing for prostate-specific antigen (PSA) and Y-chromosome DNA (Yc DNA). Results Immediately after exposure to 1000 μL of semen, the predicted sensitivity of being PSA positive was 0.96; this decreased to 0.65, 0.44, 0.21 and 0.07 at 6, 12, 24 and 48 h, respectively. Corresponding predicted sensitivity of being Yc DNA positive was 0.72 immediately postexposure; this increased to 0.76 at 1 h postexposure and then decreased to 0.60 (at 6 h), 0.63 (at 12 h), 0.49 (at 24 h), 0.21 (at 48 h), 0.17 (at 72 h) and 0.12 (at 144 h). Conclusions Overall findings suggest that PSA may be more consistent as a marker of very recent exposure and that Yc DNA is more likely to be detected in the vagina after 12 h postexposure compared to PSA.
KW - Clearance
KW - Prostate-specific antigen
KW - Semen biomarkers
KW - Y-chromosome DNA
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U2 - 10.1016/j.contraception.2013.08.003
DO - 10.1016/j.contraception.2013.08.003
M3 - Article
C2 - 24028752
AN - SCOPUS:84888015157
SN - 0010-7824
VL - 88
SP - 749
EP - 757
JO - Contraception
JF - Contraception
IS - 6
ER -