Detection of thymic surface antigens and radioactive labeling of mouse lymphoid cells

D. Allison, K. Bose, J. L. Hill

Research output: Contribution to journalArticlepeer-review

Abstract

A method for the permanent and simultaneous detection of tissue-specific surface antigens and internal radioactive labeling of mouse lymphoid cells is described. Target cells were first reacted with a mouse-derived 'antithymocyte serum', incubated with peroxidase-conjugated rabbit serum against mouse immunoglobulins and placed in substrate solution that leads to staining of the antigen-positive cells. Radioactive labeling was demonstrated by autoradiography performed after the antigen assay. More than 90% of antigen-positive thymocytes could be specifically stained in the assay without staining of similarly treated antigen-negative cells. Autoradiographic grains could be detected over both antigen-positive and antigen-negative cells.

Original languageEnglish (US)
Pages (from-to)1269-1274
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume25
Issue number11
DOIs
StatePublished - 1977

ASJC Scopus subject areas

  • Anatomy
  • Histology

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