Detection of soluble E-selectin, ICAM-1, VCAM-1, and L-selectin in the cerebrospinal fluid of patients after subarachnoid hemorrhage

Richard S. Polin, Murad Bavbek, Mark E. Shaffrey, Kevin Lenuell Billups, Christopher A. Bogaev, Neal F. Kassell, Kevin S. Lee

Research output: Contribution to journalArticle

Abstract

Object. The goal of this study was to explore whether the levels of soluble adhesion molecules were elevated in cerebrospinal fluid (CSF) after subarachnoid hemorrhage (SAH). This association was suggested by the known inflammatory response in vasospasm and the role of vascular adhesion molecules in regulating leukocytic adhesion to, and migration across, vascular endothelium. Methods. A prospective analysis was performed on CSF samples obtained in 17 patients who had suffered a recent aneurysmal SAH and in 16 control patients by using quantitative enzyme-linked immunosorbent assays for E-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular adhesion molecule- 1 (VCAM-1), and L-selectin. Levels of soluble forms of E- selectin (p = 0.0013), ICAM-1 (p = 0.0001), and VCAM-1 (p = 0.048) were found to be elevated in the CSF of patients after SAH compared with levels in the CSF of normal controls, patients with unruptured aneurysms, and patients tested months after SAH occurred. In addition, individual patients tested at the time of their initial ictus demonstrated a fall in adhesion molecule levels over time. Levels of E-selectin (p = 0.044) were highest in patients who later developed moderate or severe vasospasm. Conclusions. Adhesion molecules are known to be involved in white cell adherence to the endothelium and subsequent diapedesis and migration in which a role in initiation of tissue damage is postulated. The authors have demonstrated the elevation of three adhesion molecules, with severely elevated levels of E-selectin seen in patients who later develop vasospasm. A correlation with a role of vascular adhesion molecules in the pathogenesis of cerebral vasospasm is suggested.

Original languageEnglish (US)
Pages (from-to)559-567
Number of pages9
JournalJournal of Neurosurgery
Volume89
Issue number4
StatePublished - Oct 1998
Externally publishedYes

Fingerprint

L-Selectin
E-Selectin
Subarachnoid Hemorrhage
Intercellular Adhesion Molecule-1
Blood Vessels
Cerebrospinal Fluid
Transendothelial and Transepithelial Migration
Intracranial Vasospasm
Vascular Endothelium
Endothelium
Aneurysm
Enzyme-Linked Immunosorbent Assay

Keywords

  • E-selectin
  • Intercellular adhesion molecule-1
  • L-selectin
  • Subarachnoid hemorrhage
  • Vascular cell adhesion molecule-1

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Polin, R. S., Bavbek, M., Shaffrey, M. E., Billups, K. L., Bogaev, C. A., Kassell, N. F., & Lee, K. S. (1998). Detection of soluble E-selectin, ICAM-1, VCAM-1, and L-selectin in the cerebrospinal fluid of patients after subarachnoid hemorrhage. Journal of Neurosurgery, 89(4), 559-567.

Detection of soluble E-selectin, ICAM-1, VCAM-1, and L-selectin in the cerebrospinal fluid of patients after subarachnoid hemorrhage. / Polin, Richard S.; Bavbek, Murad; Shaffrey, Mark E.; Billups, Kevin Lenuell; Bogaev, Christopher A.; Kassell, Neal F.; Lee, Kevin S.

In: Journal of Neurosurgery, Vol. 89, No. 4, 10.1998, p. 559-567.

Research output: Contribution to journalArticle

Polin, RS, Bavbek, M, Shaffrey, ME, Billups, KL, Bogaev, CA, Kassell, NF & Lee, KS 1998, 'Detection of soluble E-selectin, ICAM-1, VCAM-1, and L-selectin in the cerebrospinal fluid of patients after subarachnoid hemorrhage', Journal of Neurosurgery, vol. 89, no. 4, pp. 559-567.
Polin, Richard S. ; Bavbek, Murad ; Shaffrey, Mark E. ; Billups, Kevin Lenuell ; Bogaev, Christopher A. ; Kassell, Neal F. ; Lee, Kevin S. / Detection of soluble E-selectin, ICAM-1, VCAM-1, and L-selectin in the cerebrospinal fluid of patients after subarachnoid hemorrhage. In: Journal of Neurosurgery. 1998 ; Vol. 89, No. 4. pp. 559-567.
@article{5854b713ab8d43098f98562f81ac2b68,
title = "Detection of soluble E-selectin, ICAM-1, VCAM-1, and L-selectin in the cerebrospinal fluid of patients after subarachnoid hemorrhage",
abstract = "Object. The goal of this study was to explore whether the levels of soluble adhesion molecules were elevated in cerebrospinal fluid (CSF) after subarachnoid hemorrhage (SAH). This association was suggested by the known inflammatory response in vasospasm and the role of vascular adhesion molecules in regulating leukocytic adhesion to, and migration across, vascular endothelium. Methods. A prospective analysis was performed on CSF samples obtained in 17 patients who had suffered a recent aneurysmal SAH and in 16 control patients by using quantitative enzyme-linked immunosorbent assays for E-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular adhesion molecule- 1 (VCAM-1), and L-selectin. Levels of soluble forms of E- selectin (p = 0.0013), ICAM-1 (p = 0.0001), and VCAM-1 (p = 0.048) were found to be elevated in the CSF of patients after SAH compared with levels in the CSF of normal controls, patients with unruptured aneurysms, and patients tested months after SAH occurred. In addition, individual patients tested at the time of their initial ictus demonstrated a fall in adhesion molecule levels over time. Levels of E-selectin (p = 0.044) were highest in patients who later developed moderate or severe vasospasm. Conclusions. Adhesion molecules are known to be involved in white cell adherence to the endothelium and subsequent diapedesis and migration in which a role in initiation of tissue damage is postulated. The authors have demonstrated the elevation of three adhesion molecules, with severely elevated levels of E-selectin seen in patients who later develop vasospasm. A correlation with a role of vascular adhesion molecules in the pathogenesis of cerebral vasospasm is suggested.",
keywords = "E-selectin, Intercellular adhesion molecule-1, L-selectin, Subarachnoid hemorrhage, Vascular cell adhesion molecule-1",
author = "Polin, {Richard S.} and Murad Bavbek and Shaffrey, {Mark E.} and Billups, {Kevin Lenuell} and Bogaev, {Christopher A.} and Kassell, {Neal F.} and Lee, {Kevin S.}",
year = "1998",
month = "10",
language = "English (US)",
volume = "89",
pages = "559--567",
journal = "Journal of Neurosurgery",
issn = "0022-3085",
publisher = "American Association of Neurological Surgeons",
number = "4",

}

TY - JOUR

T1 - Detection of soluble E-selectin, ICAM-1, VCAM-1, and L-selectin in the cerebrospinal fluid of patients after subarachnoid hemorrhage

AU - Polin, Richard S.

AU - Bavbek, Murad

AU - Shaffrey, Mark E.

AU - Billups, Kevin Lenuell

AU - Bogaev, Christopher A.

AU - Kassell, Neal F.

AU - Lee, Kevin S.

PY - 1998/10

Y1 - 1998/10

N2 - Object. The goal of this study was to explore whether the levels of soluble adhesion molecules were elevated in cerebrospinal fluid (CSF) after subarachnoid hemorrhage (SAH). This association was suggested by the known inflammatory response in vasospasm and the role of vascular adhesion molecules in regulating leukocytic adhesion to, and migration across, vascular endothelium. Methods. A prospective analysis was performed on CSF samples obtained in 17 patients who had suffered a recent aneurysmal SAH and in 16 control patients by using quantitative enzyme-linked immunosorbent assays for E-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular adhesion molecule- 1 (VCAM-1), and L-selectin. Levels of soluble forms of E- selectin (p = 0.0013), ICAM-1 (p = 0.0001), and VCAM-1 (p = 0.048) were found to be elevated in the CSF of patients after SAH compared with levels in the CSF of normal controls, patients with unruptured aneurysms, and patients tested months after SAH occurred. In addition, individual patients tested at the time of their initial ictus demonstrated a fall in adhesion molecule levels over time. Levels of E-selectin (p = 0.044) were highest in patients who later developed moderate or severe vasospasm. Conclusions. Adhesion molecules are known to be involved in white cell adherence to the endothelium and subsequent diapedesis and migration in which a role in initiation of tissue damage is postulated. The authors have demonstrated the elevation of three adhesion molecules, with severely elevated levels of E-selectin seen in patients who later develop vasospasm. A correlation with a role of vascular adhesion molecules in the pathogenesis of cerebral vasospasm is suggested.

AB - Object. The goal of this study was to explore whether the levels of soluble adhesion molecules were elevated in cerebrospinal fluid (CSF) after subarachnoid hemorrhage (SAH). This association was suggested by the known inflammatory response in vasospasm and the role of vascular adhesion molecules in regulating leukocytic adhesion to, and migration across, vascular endothelium. Methods. A prospective analysis was performed on CSF samples obtained in 17 patients who had suffered a recent aneurysmal SAH and in 16 control patients by using quantitative enzyme-linked immunosorbent assays for E-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular adhesion molecule- 1 (VCAM-1), and L-selectin. Levels of soluble forms of E- selectin (p = 0.0013), ICAM-1 (p = 0.0001), and VCAM-1 (p = 0.048) were found to be elevated in the CSF of patients after SAH compared with levels in the CSF of normal controls, patients with unruptured aneurysms, and patients tested months after SAH occurred. In addition, individual patients tested at the time of their initial ictus demonstrated a fall in adhesion molecule levels over time. Levels of E-selectin (p = 0.044) were highest in patients who later developed moderate or severe vasospasm. Conclusions. Adhesion molecules are known to be involved in white cell adherence to the endothelium and subsequent diapedesis and migration in which a role in initiation of tissue damage is postulated. The authors have demonstrated the elevation of three adhesion molecules, with severely elevated levels of E-selectin seen in patients who later develop vasospasm. A correlation with a role of vascular adhesion molecules in the pathogenesis of cerebral vasospasm is suggested.

KW - E-selectin

KW - Intercellular adhesion molecule-1

KW - L-selectin

KW - Subarachnoid hemorrhage

KW - Vascular cell adhesion molecule-1

UR - http://www.scopus.com/inward/record.url?scp=0031691441&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031691441&partnerID=8YFLogxK

M3 - Article

C2 - 9761049

AN - SCOPUS:0031691441

VL - 89

SP - 559

EP - 567

JO - Journal of Neurosurgery

JF - Journal of Neurosurgery

SN - 0022-3085

IS - 4

ER -