Detection of rupture-prone atherosclerotic plaques by time-resolved laser-induced fluorescence spectroscopy

Laura Marcu, Javier A. Jo, Qiyin Fang, Thanassis Papaioannou, Todd Reil, Jian Hua Qiao, J. Dennis Baker, Julie A. Freischlag, Michael C. Fishbein

Research output: Contribution to journalArticle

Abstract

Objective: Plaque with dense inflammatory cells, including macrophages, thin fibrous cap and superficial necrotic/lipid core is thought to be prone-to-rupture. We report a time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) technique for detection of such markers of plaque vulnerability in human plaques. Methods: The autofluorescence of carotid plaques (65 endarterectomy patients) induced by a pulsed laser (337 nm, 0.7 ns) was measured from 831 distinct areas. The emission was resolved spectrally (360-550 nm range) and temporally (0.3 ns resolution) using a prototype fiber-optic TR-LIFS apparatus. Lesions were evaluated microscopically and quantified as to the % of different components (fibrous cap, necrotic core, inflammatory cells, foam cells, mature and degraded collagen, elastic fibers, calcification, and smooth muscle cell of the vessel wall). Results: We determined that the spectral intensities and time-dependent parameters at discrete emission wavelengths (1) allow for discrimination (sensitivity >81%, specificity >94%) of various compositional and pathological features associated with plaque vulnerability including infiltration of macrophages into intima and necrotic/lipid core under a thin fibrous cap, and (2) show a linear correlation with plaque biochemical content: elastin (P <0.008), collagen (P <0.02), inflammatory cells (P <0.003), necrosis (P <0.004). Conclusion: Our results demonstrate the feasibility of TR-LIFS as a method for the identification of markers of plaque vulnerability. Current findings enable future development of TR-LIFS-based clinical devices for rapid investigation of atherosclerotic plaques and detection of those at high-risk.

Original languageEnglish (US)
Pages (from-to)156-164
Number of pages9
JournalAtherosclerosis
Volume204
Issue number1
DOIs
StatePublished - May 2009

Fingerprint

Fluorescence Spectrometry
Atherosclerotic Plaques
Rupture
Lasers
Collagen
Macrophages
Lipids
Foam Cells
Endarterectomy
Elastic Tissue
Elastin
Cell Wall
Smooth Muscle Myocytes
Necrosis
Equipment and Supplies

Keywords

  • Atherosclerosis
  • Diagnosis
  • Fluorescence spectroscopy

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Marcu, L., Jo, J. A., Fang, Q., Papaioannou, T., Reil, T., Qiao, J. H., ... Fishbein, M. C. (2009). Detection of rupture-prone atherosclerotic plaques by time-resolved laser-induced fluorescence spectroscopy. Atherosclerosis, 204(1), 156-164. https://doi.org/10.1016/j.atherosclerosis.2008.08.035

Detection of rupture-prone atherosclerotic plaques by time-resolved laser-induced fluorescence spectroscopy. / Marcu, Laura; Jo, Javier A.; Fang, Qiyin; Papaioannou, Thanassis; Reil, Todd; Qiao, Jian Hua; Baker, J. Dennis; Freischlag, Julie A.; Fishbein, Michael C.

In: Atherosclerosis, Vol. 204, No. 1, 05.2009, p. 156-164.

Research output: Contribution to journalArticle

Marcu, L, Jo, JA, Fang, Q, Papaioannou, T, Reil, T, Qiao, JH, Baker, JD, Freischlag, JA & Fishbein, MC 2009, 'Detection of rupture-prone atherosclerotic plaques by time-resolved laser-induced fluorescence spectroscopy', Atherosclerosis, vol. 204, no. 1, pp. 156-164. https://doi.org/10.1016/j.atherosclerosis.2008.08.035
Marcu, Laura ; Jo, Javier A. ; Fang, Qiyin ; Papaioannou, Thanassis ; Reil, Todd ; Qiao, Jian Hua ; Baker, J. Dennis ; Freischlag, Julie A. ; Fishbein, Michael C. / Detection of rupture-prone atherosclerotic plaques by time-resolved laser-induced fluorescence spectroscopy. In: Atherosclerosis. 2009 ; Vol. 204, No. 1. pp. 156-164.
@article{15df3b60d1914dffb7925cab8e754d84,
title = "Detection of rupture-prone atherosclerotic plaques by time-resolved laser-induced fluorescence spectroscopy",
abstract = "Objective: Plaque with dense inflammatory cells, including macrophages, thin fibrous cap and superficial necrotic/lipid core is thought to be prone-to-rupture. We report a time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) technique for detection of such markers of plaque vulnerability in human plaques. Methods: The autofluorescence of carotid plaques (65 endarterectomy patients) induced by a pulsed laser (337 nm, 0.7 ns) was measured from 831 distinct areas. The emission was resolved spectrally (360-550 nm range) and temporally (0.3 ns resolution) using a prototype fiber-optic TR-LIFS apparatus. Lesions were evaluated microscopically and quantified as to the {\%} of different components (fibrous cap, necrotic core, inflammatory cells, foam cells, mature and degraded collagen, elastic fibers, calcification, and smooth muscle cell of the vessel wall). Results: We determined that the spectral intensities and time-dependent parameters at discrete emission wavelengths (1) allow for discrimination (sensitivity >81{\%}, specificity >94{\%}) of various compositional and pathological features associated with plaque vulnerability including infiltration of macrophages into intima and necrotic/lipid core under a thin fibrous cap, and (2) show a linear correlation with plaque biochemical content: elastin (P <0.008), collagen (P <0.02), inflammatory cells (P <0.003), necrosis (P <0.004). Conclusion: Our results demonstrate the feasibility of TR-LIFS as a method for the identification of markers of plaque vulnerability. Current findings enable future development of TR-LIFS-based clinical devices for rapid investigation of atherosclerotic plaques and detection of those at high-risk.",
keywords = "Atherosclerosis, Diagnosis, Fluorescence spectroscopy",
author = "Laura Marcu and Jo, {Javier A.} and Qiyin Fang and Thanassis Papaioannou and Todd Reil and Qiao, {Jian Hua} and Baker, {J. Dennis} and Freischlag, {Julie A.} and Fishbein, {Michael C.}",
year = "2009",
month = "5",
doi = "10.1016/j.atherosclerosis.2008.08.035",
language = "English (US)",
volume = "204",
pages = "156--164",
journal = "Atherosclerosis",
issn = "0021-9150",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Detection of rupture-prone atherosclerotic plaques by time-resolved laser-induced fluorescence spectroscopy

AU - Marcu, Laura

AU - Jo, Javier A.

AU - Fang, Qiyin

AU - Papaioannou, Thanassis

AU - Reil, Todd

AU - Qiao, Jian Hua

AU - Baker, J. Dennis

AU - Freischlag, Julie A.

AU - Fishbein, Michael C.

PY - 2009/5

Y1 - 2009/5

N2 - Objective: Plaque with dense inflammatory cells, including macrophages, thin fibrous cap and superficial necrotic/lipid core is thought to be prone-to-rupture. We report a time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) technique for detection of such markers of plaque vulnerability in human plaques. Methods: The autofluorescence of carotid plaques (65 endarterectomy patients) induced by a pulsed laser (337 nm, 0.7 ns) was measured from 831 distinct areas. The emission was resolved spectrally (360-550 nm range) and temporally (0.3 ns resolution) using a prototype fiber-optic TR-LIFS apparatus. Lesions were evaluated microscopically and quantified as to the % of different components (fibrous cap, necrotic core, inflammatory cells, foam cells, mature and degraded collagen, elastic fibers, calcification, and smooth muscle cell of the vessel wall). Results: We determined that the spectral intensities and time-dependent parameters at discrete emission wavelengths (1) allow for discrimination (sensitivity >81%, specificity >94%) of various compositional and pathological features associated with plaque vulnerability including infiltration of macrophages into intima and necrotic/lipid core under a thin fibrous cap, and (2) show a linear correlation with plaque biochemical content: elastin (P <0.008), collagen (P <0.02), inflammatory cells (P <0.003), necrosis (P <0.004). Conclusion: Our results demonstrate the feasibility of TR-LIFS as a method for the identification of markers of plaque vulnerability. Current findings enable future development of TR-LIFS-based clinical devices for rapid investigation of atherosclerotic plaques and detection of those at high-risk.

AB - Objective: Plaque with dense inflammatory cells, including macrophages, thin fibrous cap and superficial necrotic/lipid core is thought to be prone-to-rupture. We report a time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) technique for detection of such markers of plaque vulnerability in human plaques. Methods: The autofluorescence of carotid plaques (65 endarterectomy patients) induced by a pulsed laser (337 nm, 0.7 ns) was measured from 831 distinct areas. The emission was resolved spectrally (360-550 nm range) and temporally (0.3 ns resolution) using a prototype fiber-optic TR-LIFS apparatus. Lesions were evaluated microscopically and quantified as to the % of different components (fibrous cap, necrotic core, inflammatory cells, foam cells, mature and degraded collagen, elastic fibers, calcification, and smooth muscle cell of the vessel wall). Results: We determined that the spectral intensities and time-dependent parameters at discrete emission wavelengths (1) allow for discrimination (sensitivity >81%, specificity >94%) of various compositional and pathological features associated with plaque vulnerability including infiltration of macrophages into intima and necrotic/lipid core under a thin fibrous cap, and (2) show a linear correlation with plaque biochemical content: elastin (P <0.008), collagen (P <0.02), inflammatory cells (P <0.003), necrosis (P <0.004). Conclusion: Our results demonstrate the feasibility of TR-LIFS as a method for the identification of markers of plaque vulnerability. Current findings enable future development of TR-LIFS-based clinical devices for rapid investigation of atherosclerotic plaques and detection of those at high-risk.

KW - Atherosclerosis

KW - Diagnosis

KW - Fluorescence spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=67349286996&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349286996&partnerID=8YFLogxK

U2 - 10.1016/j.atherosclerosis.2008.08.035

DO - 10.1016/j.atherosclerosis.2008.08.035

M3 - Article

C2 - 18926540

AN - SCOPUS:67349286996

VL - 204

SP - 156

EP - 164

JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

IS - 1

ER -