Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid

Frank M. Van Den Berg, Inge O. Baas, Mirjam M. Polak, G. Johan A Offerhaus

Research output: Contribution to journalArticlepeer-review

Abstract

With the aid of a newly developed target unmasking fluid (TUF), p53 overexpression was visualised by immunohistochemistry on recent and archival paraffin-embedded tissue samples of colon, stomach, and pancreas neoplasms. Using monoclonal anti-p53 antibody pAb 1801 as well as pofyclonal antiserum to p53 CM1, TUF-mediated immunohistochemistry was fully concordant for p53 overexpression in paraffin-embedded carcinoma samples compared with freshly frozen tissue from the same tumors. Thus, prognostic and diagnostic assessment of p53 overexpression in malignant tissue, routinely fixed in formalin and embedded in paraffin wax, by TUF-mediated immunohistochemistry with monoclonal and polyclonal antibodies may be adopted as a new tool in diagnostic and research histopathology.

Original languageEnglish (US)
Pages (from-to)381-385
Number of pages5
JournalAmerican Journal of Pathology
Volume142
Issue number2
StatePublished - Feb 1993
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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