Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid

Frank M. Van Den Berg, Inge O. Baas, Mirjam M. Polak, G. Johan A Offerhaus

Research output: Contribution to journalArticle

Abstract

With the aid of a newly developed target unmasking fluid (TUF), p53 overexpression was visualised by immunohistochemistry on recent and archival paraffin-embedded tissue samples of colon, stomach, and pancreas neoplasms. Using monoclonal anti-p53 antibody pAb 1801 as well as pofyclonal antiserum to p53 CM1, TUF-mediated immunohistochemistry was fully concordant for p53 overexpression in paraffin-embedded carcinoma samples compared with freshly frozen tissue from the same tumors. Thus, prognostic and diagnostic assessment of p53 overexpression in malignant tissue, routinely fixed in formalin and embedded in paraffin wax, by TUF-mediated immunohistochemistry with monoclonal and polyclonal antibodies may be adopted as a new tool in diagnostic and research histopathology.

Original languageEnglish (US)
Pages (from-to)381-385
Number of pages5
JournalAmerican Journal of Pathology
Volume142
Issue number2
StatePublished - Feb 1993
Externally publishedYes

Fingerprint

Paraffin
Immunohistochemistry
Carcinoma
Monoclonal Antibodies
Waxes
Pancreatic Neoplasms
Colonic Neoplasms
Formaldehyde
Stomach Neoplasms
Immune Sera
Anti-Idiotypic Antibodies
Research
Neoplasms

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid. / Van Den Berg, Frank M.; Baas, Inge O.; Polak, Mirjam M.; Offerhaus, G. Johan A.

In: American Journal of Pathology, Vol. 142, No. 2, 02.1993, p. 381-385.

Research output: Contribution to journalArticle

Van Den Berg, Frank M. ; Baas, Inge O. ; Polak, Mirjam M. ; Offerhaus, G. Johan A. / Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid. In: American Journal of Pathology. 1993 ; Vol. 142, No. 2. pp. 381-385.
@article{b104e7bf854746dda1ed90b2290c3ff1,
title = "Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid",
abstract = "With the aid of a newly developed target unmasking fluid (TUF), p53 overexpression was visualised by immunohistochemistry on recent and archival paraffin-embedded tissue samples of colon, stomach, and pancreas neoplasms. Using monoclonal anti-p53 antibody pAb 1801 as well as pofyclonal antiserum to p53 CM1, TUF-mediated immunohistochemistry was fully concordant for p53 overexpression in paraffin-embedded carcinoma samples compared with freshly frozen tissue from the same tumors. Thus, prognostic and diagnostic assessment of p53 overexpression in malignant tissue, routinely fixed in formalin and embedded in paraffin wax, by TUF-mediated immunohistochemistry with monoclonal and polyclonal antibodies may be adopted as a new tool in diagnostic and research histopathology.",
author = "{Van Den Berg}, {Frank M.} and Baas, {Inge O.} and Polak, {Mirjam M.} and Offerhaus, {G. Johan A}",
year = "1993",
month = "2",
language = "English (US)",
volume = "142",
pages = "381--385",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid

AU - Van Den Berg, Frank M.

AU - Baas, Inge O.

AU - Polak, Mirjam M.

AU - Offerhaus, G. Johan A

PY - 1993/2

Y1 - 1993/2

N2 - With the aid of a newly developed target unmasking fluid (TUF), p53 overexpression was visualised by immunohistochemistry on recent and archival paraffin-embedded tissue samples of colon, stomach, and pancreas neoplasms. Using monoclonal anti-p53 antibody pAb 1801 as well as pofyclonal antiserum to p53 CM1, TUF-mediated immunohistochemistry was fully concordant for p53 overexpression in paraffin-embedded carcinoma samples compared with freshly frozen tissue from the same tumors. Thus, prognostic and diagnostic assessment of p53 overexpression in malignant tissue, routinely fixed in formalin and embedded in paraffin wax, by TUF-mediated immunohistochemistry with monoclonal and polyclonal antibodies may be adopted as a new tool in diagnostic and research histopathology.

AB - With the aid of a newly developed target unmasking fluid (TUF), p53 overexpression was visualised by immunohistochemistry on recent and archival paraffin-embedded tissue samples of colon, stomach, and pancreas neoplasms. Using monoclonal anti-p53 antibody pAb 1801 as well as pofyclonal antiserum to p53 CM1, TUF-mediated immunohistochemistry was fully concordant for p53 overexpression in paraffin-embedded carcinoma samples compared with freshly frozen tissue from the same tumors. Thus, prognostic and diagnostic assessment of p53 overexpression in malignant tissue, routinely fixed in formalin and embedded in paraffin wax, by TUF-mediated immunohistochemistry with monoclonal and polyclonal antibodies may be adopted as a new tool in diagnostic and research histopathology.

UR - http://www.scopus.com/inward/record.url?scp=0027394094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027394094&partnerID=8YFLogxK

M3 - Article

C2 - 8434637

AN - SCOPUS:0027394094

VL - 142

SP - 381

EP - 385

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 2

ER -