Detection of ovine lentivirus in seronegative sheep by in situ hybridization, PCR, and cocultivation with susceptible cells

L. K. Johnson, A. L. Meyer, Mary Christine Zink

Research output: Contribution to journalArticle

Abstract

Serological surveys for ovine lentivirus (OvLV), a world-wide cause of pneumonia and chronic debilitation in sheep, have demonstrated a wide range of seroprevalence rates. This study analyzed OvLV infection in a purebred sheep flock with a history of OvLV disease (flock 1), and compared the prevalence with that of a flock lacking previous OvLV-associated disease (flock 2). Serological tests (ELISA and Western blot assay) indicated that 25% of sheep of all ages in flock 1 (Group A) and 33% of animals of all ages in flock 2 (Group B) had antibodies to OvLV. In situ hybridization, however, detected viral RNA in a much larger proportion of sheep (72 and 67%, respectively). Animals less than 1 year of age rarely had antibodies to OvLV, although most harbored viral RNA. Twenty animals in this age group from flock 1 (Group C) were therefore studied more closely for infection. These yearling animals were tested serologically by ELISA and their peripheral blood-derived macrophages were cultured for 14 days to amplify any infection in these target cells. The macrophages were then tested by in situ hybridization, PCR, and cocultivation with susceptible target cells. The results of these tests showed that while only 10% of animals in Group C were seropositive, 70% were positive by in situ hybridization, PCR, and cocultivation. These data suggest that latent OvLV infection is common in sheep and that infection is frequently undetected by serological tests.

Original languageEnglish (US)
Pages (from-to)254-260
Number of pages7
JournalClinical Immunology and Immunopathology
Volume65
Issue number3
DOIs
StatePublished - 1992

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Lentivirus
Coculture Techniques
In Situ Hybridization
Sheep
Polymerase Chain Reaction
Lentivirus Infections
Viral RNA
Serologic Tests
Infection
Sheep Diseases
Enzyme-Linked Immunosorbent Assay
Macrophages
Antibodies
Seroepidemiologic Studies
Pneumonia
Age Groups
Western Blotting

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Detection of ovine lentivirus in seronegative sheep by in situ hybridization, PCR, and cocultivation with susceptible cells. / Johnson, L. K.; Meyer, A. L.; Zink, Mary Christine.

In: Clinical Immunology and Immunopathology, Vol. 65, No. 3, 1992, p. 254-260.

Research output: Contribution to journalArticle

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