Detection of occult simian immunodeficiency virus SIVsmm infection in asymptomatic seronegative nonhuman primates and evidence for variation in SIV gag sequence between in vivo- and in vitro-propagated virus

F. Villinger, J. D. Powell, T. Jehuda-Cohen, N. Neckelmann, M. Vuchetich, B. De, T. M. Folks, H. M. McClure, A. A. Ansari

Research output: Contribution to journalArticlepeer-review

Abstract

Polymerase chain reaction techniques were used to identify simian immunodeficiency virus (SIV) SIVsmm gag sequences in genomic DNA isolated from peripheral blood mononuclear cells from naturally infected asymptomatic seropositive and seronegative sooty mangabeys (Cercocebus atys) and from experimentally infected but asymptomatic rhesus macaques (Macaca mulatta). The results indicate that most if not all SIV-seronegative mangabeys from the colony at the Yerkes Primate Center are in fact infected with SIVsmm despite their lack of humoral immune response, confirming previous immunological and virological observations made by our laboratory. Sequence analysis of these particular gag fragments from the mangabey revealed an average of 88% nucleotide sequence homology but 97% amino acid identity with the previously published sequence of the SIVsimmH4 clone. The significance of this finding relative to the asymptomatic state of SIV-infected mangabeys and disease-susceptible SIV-infected rhesus macaques is discussed.

Original languageEnglish (US)
Pages (from-to)1855-1862
Number of pages8
JournalJournal of virology
Volume65
Issue number4
DOIs
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Detection of occult simian immunodeficiency virus SIVsmm infection in asymptomatic seronegative nonhuman primates and evidence for variation in SIV gag sequence between in vivo- and in vitro-propagated virus'. Together they form a unique fingerprint.

Cite this