Detection of non-culturable shigella dysenteriae 1 from artificially contaminated volunteers' fingers using fluorescent antibody and.PCR techniques

M. S. Islam, M. Z. Hossain, S. I. Khan, A. Felsenstein, R. B. Sack, Andmj Albert

Research output: Contribution to journalArticlepeer-review

Abstract

Epidemiological studies have demonstrated that hands may be an important vehicle for transmission of shigellosis. The present study was carried out to find out the survival potential of Shigella dysenteriae 1 on fingers of volunteers. Finger surface was inoculated with 10s cfu of 5. dysenteriae 1 and then the bacteria were detected using conventional culture, PCR and fluorescent antibody (FA) techniques after different time intervals. It was found that 5. dysenteriae 1 survived for up to one hour in culturable form but up to four hours in non-culturable form on human fingers. The non-culturable 5. dysenteriae was detected by PCR and FA techniques. This study elaborates on the role that fingers have in the transmission of shigellae.

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
JournalJournal of diarrhoeal diseases research
Volume15
Issue number2
StatePublished - 1997

Keywords

  • Disease transmission
  • Dysentery, Bacillary
  • Flourescent antibody technique
  • Polymerase chain reaction
  • Shigella dysenteriae

ASJC Scopus subject areas

  • Gastroenterology

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