Abstract
Epidemiological studies have demonstrated that hands may be an important vehicle for transmission of shigellosis. The present study was carried out to find out the survival potential of Shigella dysenteriae 1 on fingers of volunteers. Finger surface was inoculated with 10s cfu of 5. dysenteriae 1 and then the bacteria were detected using conventional culture, PCR and fluorescent antibody (FA) techniques after different time intervals. It was found that 5. dysenteriae 1 survived for up to one hour in culturable form but up to four hours in non-culturable form on human fingers. The non-culturable 5. dysenteriae was detected by PCR and FA techniques. This study elaborates on the role that fingers have in the transmission of shigellae.
Original language | English (US) |
---|---|
Pages (from-to) | 65-70 |
Number of pages | 6 |
Journal | Journal of Diarrhoeal Diseases Research |
Volume | 15 |
Issue number | 2 |
State | Published - 1997 |
Externally published | Yes |
Keywords
- Disease transmission
- Dysentery, Bacillary
- Flourescent antibody technique
- Polymerase chain reaction
- Shigella dysenteriae
ASJC Scopus subject areas
- Gastroenterology