Detection of nicotine and nicotine metabolites in Units of banked blood

Joesph R. Wiencek, Eric A. Gehrie, Amaris M. Keiser, Penny C. Szklarski, Kamisha L. Johnson-Davis, Garrett S. Booth

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Objectives To determine the concentrations of nicotine and nicotine metabolites in RBC units as a means to estimate the point prevalence of exposure within the healthy donor pool. Methods Segments from 105 RBC units were tested for the presence of nicotine, cotinine, or trans-3' -hydroxycotinine by liquid chromatography-tandem mass spectrometry. Results Of the 20 (19%) units that contained detectable concentrations of nicotine, cotinine, or trans-3' -hydroxycotinine, 19 (18.1%) contained concentrations consistent with the use of a nicotine-containing product within 48 hours of specimen collection. One RBC unit contained nicotine concentrations consistent with passive exposure. Conclusions Chemicals from nicotine-containing products are detectable within the US RBC supply. Further investigation is needed to determine the risks of transfusion-associated exposure to nicotine and other tobacco-associated chemicals among vulnerable patient populations such as neonates.

Original languageEnglish (US)
Pages (from-to)516-521
Number of pages6
JournalAmerican journal of clinical pathology
Issue number5
StatePublished - Apr 2 2019

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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