Detection of malaria liver-stages in mice infected through the bite of a single Anopheles mosquito using a highly sensitive real-time PCR

Oscar Brua-Romero, Julius C R Hafalla, Gloria González-Aseguinolaza, Gen ichiro Sano, Moriya Tsuji, Fidel P Zavala

Research output: Contribution to journalArticle

Abstract

We describe a highly sensitive real-time PCR to detect and measure the development of the liver-stages of malaria parasites in mice infected with sporozoites ranging in number from 25 to more than 164,000, using the same reaction conditions. Furthermore, this assay detects and measures parasite loads in the livers of mice exposed to the bite of a single malaria-infected Anopheles mosquito. This unique method should greatly facilitate studies aimed at evaluating very precisely the efficacy of anti-malarial experimental drug treatments and vaccination regimens in conditions of infection resembling those found in the field.

Original languageEnglish (US)
Pages (from-to)1499-1502
Number of pages4
JournalInternational Journal for Parasitology
Volume31
Issue number13
DOIs
StatePublished - 2001
Externally publishedYes

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Anopheles
Bites and Stings
Culicidae
Malaria
Real-Time Polymerase Chain Reaction
Parasite Load
Sporozoites
Liver
Antimalarials
Parasites
Vaccination
Infection
Pharmaceutical Preparations
Therapeutics

Keywords

  • Liver-stages
  • Malaria
  • Plasmodium
  • Real-time PCR

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Detection of malaria liver-stages in mice infected through the bite of a single Anopheles mosquito using a highly sensitive real-time PCR. / Brua-Romero, Oscar; Hafalla, Julius C R; González-Aseguinolaza, Gloria; Sano, Gen ichiro; Tsuji, Moriya; Zavala, Fidel P.

In: International Journal for Parasitology, Vol. 31, No. 13, 2001, p. 1499-1502.

Research output: Contribution to journalArticle

Brua-Romero, Oscar ; Hafalla, Julius C R ; González-Aseguinolaza, Gloria ; Sano, Gen ichiro ; Tsuji, Moriya ; Zavala, Fidel P. / Detection of malaria liver-stages in mice infected through the bite of a single Anopheles mosquito using a highly sensitive real-time PCR. In: International Journal for Parasitology. 2001 ; Vol. 31, No. 13. pp. 1499-1502.
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