Detection of JC Virus-Specific Immune Responses in a Novel Humanized Mouse Model

Chen Sabrina Tan, Thomas A. Broge, Edward Seung, Vlad Vrbanac, Raphael P Viscidi, Jennifer Gordon, Andrew M. Tager, Igor J. Koralnik

Research output: Contribution to journalArticle

Abstract

Progressive Multifocal Leukoencephalopathy (PML) is an often fatal disease caused by the reactivation of the JC virus (JCV). Better understanding of viral-host interactions has been hampered by the lack of an animal model. Engrafting NOD/SCID/IL-2-Rg (null) mice with human lymphocytes and thymus, we generated a novel animal model for JCV infection. Mice were inoculated with either a PML isolate, JCV Mad-4, or with JCV CY, found in the kidney and urine of healthy individuals. While mice remained asymptomatic following inoculation, JCV DNA was occasionally detected in both the blood and the urine compartments. Mice generated both humoral and cellular immune responses against JCV. Expressions of immune exhaustion marker, PD-1, on lymphocytes were consistent with response to infection. Using this model we present the first in vivo demonstration of virological and immunological differences between JCV Mad-4 and CY. This model may prove valuable for studying JCV host immune responses.

Original languageEnglish (US)
Article numbere64313
JournalPLoS One
Volume8
Issue number5
DOIs
StatePublished - May 20 2013

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JC polyomavirus
JC Virus
Viruses
animal models
immune response
Progressive Multifocal Leukoencephalopathy
Lymphocytes
mice
Animals
urine
lymphocytes
Animal Models
Urine
Thymus
Virus Diseases
Humoral Immunity
interleukin-2
humoral immunity
Cellular Immunity
infection

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tan, C. S., Broge, T. A., Seung, E., Vrbanac, V., Viscidi, R. P., Gordon, J., ... Koralnik, I. J. (2013). Detection of JC Virus-Specific Immune Responses in a Novel Humanized Mouse Model. PLoS One, 8(5), [e64313]. https://doi.org/10.1371/journal.pone.0064313

Detection of JC Virus-Specific Immune Responses in a Novel Humanized Mouse Model. / Tan, Chen Sabrina; Broge, Thomas A.; Seung, Edward; Vrbanac, Vlad; Viscidi, Raphael P; Gordon, Jennifer; Tager, Andrew M.; Koralnik, Igor J.

In: PLoS One, Vol. 8, No. 5, e64313, 20.05.2013.

Research output: Contribution to journalArticle

Tan, CS, Broge, TA, Seung, E, Vrbanac, V, Viscidi, RP, Gordon, J, Tager, AM & Koralnik, IJ 2013, 'Detection of JC Virus-Specific Immune Responses in a Novel Humanized Mouse Model', PLoS One, vol. 8, no. 5, e64313. https://doi.org/10.1371/journal.pone.0064313
Tan, Chen Sabrina ; Broge, Thomas A. ; Seung, Edward ; Vrbanac, Vlad ; Viscidi, Raphael P ; Gordon, Jennifer ; Tager, Andrew M. ; Koralnik, Igor J. / Detection of JC Virus-Specific Immune Responses in a Novel Humanized Mouse Model. In: PLoS One. 2013 ; Vol. 8, No. 5.
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