TY - JOUR
T1 - Detection of intrauterine viral infection using the polymerase chain reaction
AU - Van Den Veyver, Ignatia B.
AU - Ni, Jiyuan
AU - Bowles, Neil
AU - Carpenter, Robert J.
AU - Weiner, Carl P.
AU - Yankowitz, Jerome
AU - Moise, Kenneth J.
AU - Henderson, Janice
AU - Towbin, Jeffrey A.
N1 - Funding Information:
This work was performed in the Phoebe Willingham Muzzy Pediatric Molecular Cardiology Laboratory. The authors are indebted to the physicians who referred samples for study. The work was supported in part by grants from the United States Public Health Service, HL49401 (CPW) and HL51735 (CPW).
PY - 1998/2
Y1 - 1998/2
N2 - Intrauterine viral infection commonly presents as nonimmune hydrops fetalis or intrauterine growth restriction. Cytomegalovirus (CMV) and parvovirus are commonly recognized causes of fetal infection using serology and cultures. We used the polymerase chain reaction (PCR) to evaluate the frequency of fetal vital infection and the associated clinical course and outcome. Specimens (amniotic fluid, fetal blood, pleural fluid, tissue) from 303 abnormal pregnancies at risk for vital infection and 154 controls were analyzed using primers for CMV, herpes simplex virus, parvovirus B19, adenovirus, enterovirus, Epstein-Barr virus, and respiratory syncytial virus. Viral genome was detected in 144/371 samples (39%) or 124/303 patients (41%), with adenovirus (n = 74 patients; 24%), CMV (n = 30 patients; 10%), and enterovirus (n = 22 patients; 7%) most common. Only 4/154 (2.6%), unaffected control patients' samples were PCR positive. We conclude that diagnosis of fetal vital infection by PCR is common in abnormal pregnancies. Adenovirus and enterovirus may cause fetal infection that have been previously unrecognized.
AB - Intrauterine viral infection commonly presents as nonimmune hydrops fetalis or intrauterine growth restriction. Cytomegalovirus (CMV) and parvovirus are commonly recognized causes of fetal infection using serology and cultures. We used the polymerase chain reaction (PCR) to evaluate the frequency of fetal vital infection and the associated clinical course and outcome. Specimens (amniotic fluid, fetal blood, pleural fluid, tissue) from 303 abnormal pregnancies at risk for vital infection and 154 controls were analyzed using primers for CMV, herpes simplex virus, parvovirus B19, adenovirus, enterovirus, Epstein-Barr virus, and respiratory syncytial virus. Viral genome was detected in 144/371 samples (39%) or 124/303 patients (41%), with adenovirus (n = 74 patients; 24%), CMV (n = 30 patients; 10%), and enterovirus (n = 22 patients; 7%) most common. Only 4/154 (2.6%), unaffected control patients' samples were PCR positive. We conclude that diagnosis of fetal vital infection by PCR is common in abnormal pregnancies. Adenovirus and enterovirus may cause fetal infection that have been previously unrecognized.
KW - Fetal infection
KW - Nonimmune hydrops
KW - Polymerase chain reaction (PCR)
KW - Viral infection
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U2 - 10.1006/mgme.1997.2651
DO - 10.1006/mgme.1997.2651
M3 - Article
C2 - 9562961
AN - SCOPUS:0031684097
SN - 1096-7192
VL - 63
SP - 85
EP - 95
JO - Molecular genetics and metabolism
JF - Molecular genetics and metabolism
IS - 2
ER -