Detection of IL-1α and IL-1β in the supernatants of paraformaldehyde-treated human monocytes. Evidence against a membrane form of IL-1

J. Suttles, L. M. Carruth, S. B. Mizel

Research output: Contribution to journalArticlepeer-review

Abstract

The concept of a membrane form of IL-1 arose from the observation that paraformaldehyde-treated macrophages display IL-1 bioactivity. Thus far, the biochemical characterization of a membrane form of the molecule has not been reported. In a recent publication we demonstrated that murine IL-1α can be detected in the supernatants of paraformaldehyde-treated macrophages. These data indicate that the phenomenon of membrane IL-1 may result from leakage of IL-1 from inadequately fixed cells. In the current report we have extended our studies toward the examination of human IL-1α and IL-1β. IL-1 activity can be detected in the supernatants of paraformaldehyde-treated human monocytes. Although anti-IL-1α, but not anti-IL-1β, antibodies can efficiently block the IL-1 bioactivity, both IL-1α and IL-1β can be found by immunoprecipitation in the supernatants of the fixed monocytes. IL-1α is efficiently processed to the low m.w. form, whereas IL-1β remains predominantly as the inactive, precursor molecule. IL-1 is not found in the supernatants of monocyte membrane preparations, demonstrating that the leakage of IL-1 is from an intracellular, rather than membrane-bound source.

Original languageEnglish (US)
Pages (from-to)170-174
Number of pages5
JournalJournal of Immunology
Volume144
Issue number1
StatePublished - 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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