Detection of human papovaviruses BKV and JCV in urines by ELISA.

R. R. Arthur, K. V. Shah, R. H. Yolken, P. Charache

Research output: Contribution to journalArticle

Abstract

A double-antibody indirect ELISA using a chromogenic substrate and a single-antibody sandwich direct ELISA using chromogenic and fluorogenic substrates were developed for the detection of the human papovaviruses BKV and JCV. All three assays were comparable in their sensitivity. BKV was detected in urine from 12 of 63 (19%) highly immunocompromised patients and JCV in urine from 4 of 31 (12.9%) patients by the indirect method.

Original languageEnglish (US)
Pages (from-to)169-176
Number of pages8
JournalProgress in clinical and biological research
Volume105
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Medicine(all)

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