Detection of human papillomavirus in the prostate by polymerase chain reaction and in situ hybridization

G. K. Ibrahim, P. E. Gravitt, K. L. Dittrich, S. N. Ibrahim, O. Melhus, S. M. Anderson, C. N. Robertson

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Human papillomavirus is associated with a variety of anogenital lesions, including genital warts, precancers and cancers. In male patients human papillomavirus has been identified in proliferative lesions ranging from penile and urethral warts to penile and prostatic cancers. We examined the association of human papillomavirus deoxyribonucleic acid (DNA) in 84 prostate tissue specimens. Specimens were selected from radical prostatectomy, transurethral resection or transrectal biopsy procedures. A total of 60 formalin-fixed, paraffin-embedded tissues (24 prostate cancer specimens, 16 benign prostatic hyperplasia specimens and 20 normal specimens) was examined by polymerase chain reaction and in situ hybridization. Also, 24 gelatin-embedded frozen prostate cancer specimens were examined for human papillomavirus DNA by polymerase chain reaction. Of the specimens 69 were deemed adequate for polymerase chain reaction analysis, whereas all 60 paraffin-embedded tissues were sufficient for in situ hybridization. Human papillomavirus DNA was detected in 2 normal tissues and 6 prostate cancers using polymerase chain reaction. None of the benign prostatic hyperplasia specimens was positive for human papillomavirus. Human papillomavirus typing results indicated that virus type 16 was present in each of the 8 positive specimens. Confirmation of the presence of human papillomavirus was obtained for 1 of the prostate cancers by nonisotopic in situ hybridization with biotinylated human papillomavirus genomic probes. The low prevalence of human papillomavirus in this study population does not strongly support an etiological role for the virus in prostate cancer.

Original languageEnglish (US)
Pages (from-to)1822-1826
Number of pages5
JournalJournal of Urology
Issue number6
StatePublished - 1992
Externally publishedYes


  • hybridization
  • papillomaviruses
  • prostatic hypertrophy
  • prostatic neoplasms

ASJC Scopus subject areas

  • Urology


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