Detection of human immunodeficiency virus regulatory protein tat mRNA in CNS tissues

Jian Kai Liu, Xiao Dong Zhao, Carol Martin, Avindra Nath

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To determine the role of Tat in mediating HIV encephalitis (HIVE). Methods: The contents of tat mRNA in tissue extracts of 9 AIDS patients with HIVE and 7 AIDS patients without HIVE were measured with RT-PCR. Results: Despite long autopsy times and significant degradation of RNA extracted from brain tissue, tat mRNA was detected in 4/9 patients with HIVE but not in any of the 7 patients without HIVE. Similarly, the env mRNA was also detected in 5/9 patients with HIVE but not in the patients without HIVE. However, vif mRNA was detected in both groups of patients with HIVE (5/9) or without HIVE (2/7). Conclusion: This result suggests that Tat likely plays an important role in the neuropathogenesis of HIVE.

Original languageEnglish (US)
Pages (from-to)223-226
Number of pages4
JournalJournal of Jilin University Medicine Edition
Volume31
Issue number2
StatePublished - Mar 28 2005

Keywords

  • AIDS dementia complex
  • Reverse transcriptase polymerase chain reaction
  • Southern blotting
  • Tat

ASJC Scopus subject areas

  • Pharmacology

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