Detection of HLA class I-specific antibodies by the Quikscreen enzyme- linked immunosorbent assay

Donna P. Lucas, Millie L. Paparounis, Leslie Myers, John M. Hart, Andrea A. Zachary

Research output: Contribution to journalArticle

Abstract

The GTI QuikScreen test is an enzyme-linked immunosorbent assay (ELISA) that uses soluble HLA class I antigens as targets. In tests of 5,893 human serum specimens, we evaluated the reliability, sensitivity, and utility of the GTI QuikScreen test for detecting HLA class 1-specific antibody. We found that the test could reliably detect HLA-specific antibodies of the immunoglobulin G (IgG) but not the IgM class. The degree of correlation with lymphocytotoxicity testing varied among the different serum sources, with the best correlation achieved with sera from renal transplant candidates (r > 0.7) and the poorest with sera from patients with end-stage liver disease (r = 0.26), possibly because of elevated alkaline phosphatase levels in the liver patients. Test reproducibility was high (96%), and test failure rate was low (1.7%). The test sensitivity is comparable to that of the antiglobulin cytotoxicity and, possibly, even flow cytometric tests. There was a highly significant (P <0.001) correlation between the optical densities obtained in the ELISA and the percent panel reactive antibody determined by cytotoxicity testing. Therefore, although designed only to determine the presence or absence of HLA-specific antibody, GTI QuikScreen test results also provided an indication of the extent of sensitization. The test is one of the most effective and efficient ways to determine if antibodies producing a positive result in crossmatch tests are specific for HLA class I antigens. As an adjunct to serum screening by cytotoxicity testing, the GTI QuikScreen test can produce a substantial savings of time and effort that reduces the cost to the laboratory and to the patient.

Original languageEnglish (US)
Pages (from-to)252-257
Number of pages6
JournalClinical and Diagnostic Laboratory Immunology
Volume4
Issue number3
StatePublished - May 1997

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Immunosorbents
Assays
Enzyme-Linked Immunosorbent Assay
Cytotoxicity
Antibodies
Enzymes
Serum
Histocompatibility Antigens Class I
HLA Antigens
Liver
Testing
Transplants
End Stage Liver Disease
Density (optical)
Alkaline Phosphatase
Immunoglobulin M
Anti-Idiotypic Antibodies
Screening
Immunoglobulin G
Kidney

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

Lucas, D. P., Paparounis, M. L., Myers, L., Hart, J. M., & Zachary, A. A. (1997). Detection of HLA class I-specific antibodies by the Quikscreen enzyme- linked immunosorbent assay. Clinical and Diagnostic Laboratory Immunology, 4(3), 252-257.

Detection of HLA class I-specific antibodies by the Quikscreen enzyme- linked immunosorbent assay. / Lucas, Donna P.; Paparounis, Millie L.; Myers, Leslie; Hart, John M.; Zachary, Andrea A.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 4, No. 3, 05.1997, p. 252-257.

Research output: Contribution to journalArticle

Lucas, DP, Paparounis, ML, Myers, L, Hart, JM & Zachary, AA 1997, 'Detection of HLA class I-specific antibodies by the Quikscreen enzyme- linked immunosorbent assay', Clinical and Diagnostic Laboratory Immunology, vol. 4, no. 3, pp. 252-257.
Lucas, Donna P. ; Paparounis, Millie L. ; Myers, Leslie ; Hart, John M. ; Zachary, Andrea A. / Detection of HLA class I-specific antibodies by the Quikscreen enzyme- linked immunosorbent assay. In: Clinical and Diagnostic Laboratory Immunology. 1997 ; Vol. 4, No. 3. pp. 252-257.
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