Detection of Helicobacter pylori using nested polymerase chain reaction in gastric biopsy samples

Divya Mahajan, Anju Jain, Varsha Singh, A. K. Jain, G. R.K. Rao, Gopal Nath

Research output: Contribution to journalArticle

Abstract

Helicobacter pylori remains a controversial organism with regards to humans, its epidemiology still unclear nearly two decades after discovery. The present study was undertaken to estimate the prevalence of the organism in the gastrointestinal tract in symptomatic and asymptomatic subjects to understand its precise natural history in India. A total of 154 specimens were a part of the study. These included gastric biopsies from peptic ulcer disease and Non ulcer dyspepsia subjects, as visualized on endoscopy, saliva and stool samples from apparently normal healthy adults. Nested polymerase chain reaction was performed using the primers Hp1, Hp2, Hp3 targeting 16S rRNA gene. A prevalence of 65.1%, 100%, 66.7%, and 73.3% respectively was observed by polymerase chain reaction. No association was observed between the H.pylori status and the disease condition of the patient.

Original languageEnglish (US)
Pages (from-to)243-245
Number of pages3
JournalIndian Journal of Clinical Biochemistry
Volume23
Issue number3
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

Keywords

  • Helicobacter pylori
  • Non ulcer dyspepsia
  • Peptic ulcer disease
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Clinical Biochemistry

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