Detection of estrogen receptor in breast and endometrial carcinoma by the immunoperoxidase technique

C. R. Taylor, C. L. Cooper, Robert J Kurman, U. Goebelsmann, F. S. Markland

Research output: Contribution to journalArticle

Abstract

Paraffin-embedded tissues from 15 women with breast or endometrial carcinomas were analyzed in a study designed to explore the possible value and validity of an immunoperoxidase method for the detection of estrogen receptor in formalin-fixed paraffin-embedded tissue. Results were compared with biochemical assays for estrogen receptor performed on cytosols of fresh tissue from the same patients. There was complete correlation in nine of 15 (60%) of the tumors analyzed. Four cases (26.7%) were judged positive for estrogen binding sites by immunoperoxidase but were negative for estrogen receptor by biochemical assays; in two cases the converse was observed. The immunohistologic technique is relatively rapid and utilizes fixed paraffin sections of the same tissue that is used for standard histologic diagnosis. The provision of a permanent record that can be kept for future reference provides an advantage over immunofluorescence methods that have been advanced for the detection of estrogen receptors. The excellent morphology achieved permits an assessment of the staining of individual cells in relation to the usual histologic criteria employed in the diagnosis of breast and endometrial cancer. This stands in contrast to biochemical cytosol-based assays that take no account of variations in receptor expression by the tumor cells or variations in the proportion of the assayed material that is in fact neoplastic as distinct from supporting stroma and connective tissue.

Original languageEnglish (US)
Pages (from-to)2634-2640
Number of pages7
JournalCancer
Volume47
Issue number11
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

Endometrial Neoplasms
Immunoenzyme Techniques
Estrogen Receptors
Paraffin
Breast Neoplasms
Cytosol
Connective Tissue
Formaldehyde
Fluorescent Antibody Technique
Neoplasms
Estrogens
Binding Sites
Staining and Labeling

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Detection of estrogen receptor in breast and endometrial carcinoma by the immunoperoxidase technique. / Taylor, C. R.; Cooper, C. L.; Kurman, Robert J; Goebelsmann, U.; Markland, F. S.

In: Cancer, Vol. 47, No. 11, 1981, p. 2634-2640.

Research output: Contribution to journalArticle

Taylor, C. R. ; Cooper, C. L. ; Kurman, Robert J ; Goebelsmann, U. ; Markland, F. S. / Detection of estrogen receptor in breast and endometrial carcinoma by the immunoperoxidase technique. In: Cancer. 1981 ; Vol. 47, No. 11. pp. 2634-2640.
@article{cbf5b257c93f4dcb835976bb6a8ab43b,
title = "Detection of estrogen receptor in breast and endometrial carcinoma by the immunoperoxidase technique",
abstract = "Paraffin-embedded tissues from 15 women with breast or endometrial carcinomas were analyzed in a study designed to explore the possible value and validity of an immunoperoxidase method for the detection of estrogen receptor in formalin-fixed paraffin-embedded tissue. Results were compared with biochemical assays for estrogen receptor performed on cytosols of fresh tissue from the same patients. There was complete correlation in nine of 15 (60{\%}) of the tumors analyzed. Four cases (26.7{\%}) were judged positive for estrogen binding sites by immunoperoxidase but were negative for estrogen receptor by biochemical assays; in two cases the converse was observed. The immunohistologic technique is relatively rapid and utilizes fixed paraffin sections of the same tissue that is used for standard histologic diagnosis. The provision of a permanent record that can be kept for future reference provides an advantage over immunofluorescence methods that have been advanced for the detection of estrogen receptors. The excellent morphology achieved permits an assessment of the staining of individual cells in relation to the usual histologic criteria employed in the diagnosis of breast and endometrial cancer. This stands in contrast to biochemical cytosol-based assays that take no account of variations in receptor expression by the tumor cells or variations in the proportion of the assayed material that is in fact neoplastic as distinct from supporting stroma and connective tissue.",
author = "Taylor, {C. R.} and Cooper, {C. L.} and Kurman, {Robert J} and U. Goebelsmann and Markland, {F. S.}",
year = "1981",
doi = "10.1002/1097-0142(19810601)47:11<2634::AID-CNCR2820471119>3.0.CO;2-H",
language = "English (US)",
volume = "47",
pages = "2634--2640",
journal = "Cancer",
issn = "0008-543X",
publisher = "John Wiley and Sons Inc.",
number = "11",

}

TY - JOUR

T1 - Detection of estrogen receptor in breast and endometrial carcinoma by the immunoperoxidase technique

AU - Taylor, C. R.

AU - Cooper, C. L.

AU - Kurman, Robert J

AU - Goebelsmann, U.

AU - Markland, F. S.

PY - 1981

Y1 - 1981

N2 - Paraffin-embedded tissues from 15 women with breast or endometrial carcinomas were analyzed in a study designed to explore the possible value and validity of an immunoperoxidase method for the detection of estrogen receptor in formalin-fixed paraffin-embedded tissue. Results were compared with biochemical assays for estrogen receptor performed on cytosols of fresh tissue from the same patients. There was complete correlation in nine of 15 (60%) of the tumors analyzed. Four cases (26.7%) were judged positive for estrogen binding sites by immunoperoxidase but were negative for estrogen receptor by biochemical assays; in two cases the converse was observed. The immunohistologic technique is relatively rapid and utilizes fixed paraffin sections of the same tissue that is used for standard histologic diagnosis. The provision of a permanent record that can be kept for future reference provides an advantage over immunofluorescence methods that have been advanced for the detection of estrogen receptors. The excellent morphology achieved permits an assessment of the staining of individual cells in relation to the usual histologic criteria employed in the diagnosis of breast and endometrial cancer. This stands in contrast to biochemical cytosol-based assays that take no account of variations in receptor expression by the tumor cells or variations in the proportion of the assayed material that is in fact neoplastic as distinct from supporting stroma and connective tissue.

AB - Paraffin-embedded tissues from 15 women with breast or endometrial carcinomas were analyzed in a study designed to explore the possible value and validity of an immunoperoxidase method for the detection of estrogen receptor in formalin-fixed paraffin-embedded tissue. Results were compared with biochemical assays for estrogen receptor performed on cytosols of fresh tissue from the same patients. There was complete correlation in nine of 15 (60%) of the tumors analyzed. Four cases (26.7%) were judged positive for estrogen binding sites by immunoperoxidase but were negative for estrogen receptor by biochemical assays; in two cases the converse was observed. The immunohistologic technique is relatively rapid and utilizes fixed paraffin sections of the same tissue that is used for standard histologic diagnosis. The provision of a permanent record that can be kept for future reference provides an advantage over immunofluorescence methods that have been advanced for the detection of estrogen receptors. The excellent morphology achieved permits an assessment of the staining of individual cells in relation to the usual histologic criteria employed in the diagnosis of breast and endometrial cancer. This stands in contrast to biochemical cytosol-based assays that take no account of variations in receptor expression by the tumor cells or variations in the proportion of the assayed material that is in fact neoplastic as distinct from supporting stroma and connective tissue.

UR - http://www.scopus.com/inward/record.url?scp=0019504550&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019504550&partnerID=8YFLogxK

U2 - 10.1002/1097-0142(19810601)47:11<2634::AID-CNCR2820471119>3.0.CO;2-H

DO - 10.1002/1097-0142(19810601)47:11<2634::AID-CNCR2820471119>3.0.CO;2-H

M3 - Article

C2 - 6167343

AN - SCOPUS:0019504550

VL - 47

SP - 2634

EP - 2640

JO - Cancer

JF - Cancer

SN - 0008-543X

IS - 11

ER -