Detection of Escherichia coli enterotoxins in stools

M. H. Merson, R. H. Yolken, R. B. Sack, J. L. Froehlich, H. B. Greenberg, I. Huq, R. W. Black

Research output: Contribution to journalArticle

Abstract

We determined whether enterotoxigenic Escherichia coli diarrhea could be diagnosed by direct examination of stools for heat-labile (LT) and heat-stable (ST) enterotoxins. The Y-1 adrenal cell and an enzyme-linked immunosorbent assay (ELISA) detected LT in 85 and 93%, respectively, of stool specimens obtained from adults with acute diarrhea from whom an LT- and ST-producing organism had been isolated. Furthermore, the ELISA assay detected LT in 8 of 35 stool specimens from which no LT-producing E. coli had been isolated. The infant mouse assay was utilized to detect ST in these stool specimens and was found to be an insensitive method, showing positive results in only 36% of the specimens from which an ST-producing organism was isolated. Further studies are warranted to determine the diagnostic value of direct detection of LT in stools, especially by the ELISA method.

Original languageEnglish (US)
Pages (from-to)108-113
Number of pages6
JournalInfection and Immunity
Volume29
Issue number1
StatePublished - 1980

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Enterotoxins
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Diarrhea
Hot Temperature
Enterotoxigenic Escherichia coli

ASJC Scopus subject areas

  • Immunology

Cite this

Merson, M. H., Yolken, R. H., Sack, R. B., Froehlich, J. L., Greenberg, H. B., Huq, I., & Black, R. W. (1980). Detection of Escherichia coli enterotoxins in stools. Infection and Immunity, 29(1), 108-113.

Detection of Escherichia coli enterotoxins in stools. / Merson, M. H.; Yolken, R. H.; Sack, R. B.; Froehlich, J. L.; Greenberg, H. B.; Huq, I.; Black, R. W.

In: Infection and Immunity, Vol. 29, No. 1, 1980, p. 108-113.

Research output: Contribution to journalArticle

Merson, MH, Yolken, RH, Sack, RB, Froehlich, JL, Greenberg, HB, Huq, I & Black, RW 1980, 'Detection of Escherichia coli enterotoxins in stools', Infection and Immunity, vol. 29, no. 1, pp. 108-113.
Merson MH, Yolken RH, Sack RB, Froehlich JL, Greenberg HB, Huq I et al. Detection of Escherichia coli enterotoxins in stools. Infection and Immunity. 1980;29(1):108-113.
Merson, M. H. ; Yolken, R. H. ; Sack, R. B. ; Froehlich, J. L. ; Greenberg, H. B. ; Huq, I. ; Black, R. W. / Detection of Escherichia coli enterotoxins in stools. In: Infection and Immunity. 1980 ; Vol. 29, No. 1. pp. 108-113.
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