Detection of elevated levels of circulating antigen 85 by dot immunobinding assay in captive wild animals with tuberculosis

Barbara J. Mangold, Robert A. Cook, Michael R. Cranfield, Kris Huygen, Henry P. Godfrey

Research output: Contribution to journalArticle

Abstract

Antemortem diagnosis of tuberculosis in captive wild animals is often difficult. In addition to the variability of host cellular immune response, which does not always indicate current active infection, reactivity to saprophytic or other mycobacteria is common and may interfere with the interpretation of the intradermal tuberculin skin test. Furthermore the immobilization required for administering the test and evaluating skin reactions in these animals may result in unacceptable levels of morbidity and mortality, of particular concern in individuals of rare or endangered species. Proteins of the antigen 85 (Ag85) complex are major secretory products of actively metabolizing mycobacteria in vitro Production of these proteins by mycobacteria during growth in vivo could result in increases in circulating levels of Ag85 in hosts with active tuberculosis. A dot blot immunoassay has been used to detect and quantify circulating Ag85 in captive wild animals with tuberculosis. Elevated levels of Ag85 were observed in animals with active tuberculosis as compared with uninfected animals. Study populations included a herd of nyala (Tragelaphus angasi) (n = 9) with no history of exposure to Mycobacterium bovis. Serum Ag85 levels ranged from

Original languageEnglish (US)
Pages (from-to)477-483
Number of pages7
JournalJournal of Zoo and Wildlife Medicine
Volume30
Issue number4
StatePublished - Dec 1999

Fingerprint

circulating antigens
captive animals
Wild Animals
tuberculosis
wild animals
antigen
Mycobacterium
Tuberculosis
assay
antigens
Antigens
assays
Skin Tests
Tragelaphus
animal
skin
animals
tuberculin
skin tests
Mycobacterium bovis

Keywords

  • Antigen 85
  • Fibronectin binding
  • Immunoassay
  • Tuberculosis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Environmental Science(all)
  • veterinary(all)

Cite this

Detection of elevated levels of circulating antigen 85 by dot immunobinding assay in captive wild animals with tuberculosis. / Mangold, Barbara J.; Cook, Robert A.; Cranfield, Michael R.; Huygen, Kris; Godfrey, Henry P.

In: Journal of Zoo and Wildlife Medicine, Vol. 30, No. 4, 12.1999, p. 477-483.

Research output: Contribution to journalArticle

Mangold, Barbara J. ; Cook, Robert A. ; Cranfield, Michael R. ; Huygen, Kris ; Godfrey, Henry P. / Detection of elevated levels of circulating antigen 85 by dot immunobinding assay in captive wild animals with tuberculosis. In: Journal of Zoo and Wildlife Medicine. 1999 ; Vol. 30, No. 4. pp. 477-483.
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