Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor

Jae Sung Yu, Hua Xin Liao, Aren E. Gerdon, Brian Huffman, Richard M. Scearce, Mille McAdams, S. Munir Alam, Paul M. Popernack, Nancy J. Sullivan, David Wright, David E. Cliffel, Gary J. Nabel, Barton F. Haynes

Research output: Contribution to journalArticle

Abstract

Ebola virus (EBOV) Zaire, Sudan, as well as Ivory Coast are virulent human EBOV species. Both polyclonal and monoclonal antibodies (MAbs) were developed against soluble EBOV envelope glycoprotein (GP) for the study of EBOV envelope diversity and development of diagnostic reagents. Three EBOV Sudan-Gulu GP peptides, from the N-terminus, mid-GP, and C-terminus regions were used to immunize rabbits for the generation of anti-EBOV polyclonal antibodies. Polyclonal antisera raised against the C-terminus peptide could detect both Sudan-Gulu as well as Zaire GPs, while anti-N and mid-region peptide polyclonal sera recognized only EBOV Sudan-Gulu GP. Of the three anti-EBOV GP mouse MAbs produced, MAb 15H10 recognized all human EBOV GP species tested (Zaire, Sudan and Ivory Coast), and as well as reacted with the Reston non-human primate EBOV GPs. In addition, MAb 15H10 bound virion-associated GP of all known EBOV species. MAb 17A3 recognized GPs of both EBOV Sudan-Gulu and Zaire, while MAb 6D11 recognized only EBOV Sudan-Gulu GP. To detect EBOV GP, these antibody reagents were used in ELISA, surface plasmon resonance and in a quartz crystal microbalance immunosensor. Thus, polyclonal and monoclonal antibodies can be used in combination to identify and differentiate both human and non-human primate EBOV GPs.

Original languageEnglish (US)
Pages (from-to)219-228
Number of pages10
JournalJournal of Virological Methods
Volume137
Issue number2
DOIs
StatePublished - Nov 2006
Externally publishedYes

Fingerprint

Ebolavirus
Quartz Crystal Microbalance Techniques
Surface Plasmon Resonance
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Glycoproteins
Cote d'Ivoire
Democratic Republic of the Congo
Sudan
Peptides
Primates
Antibodies

Keywords

  • Ebola virus
  • Glycoprotein detection
  • Monoclonal antibody
  • Quartz crystal microbalance immunosensor
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Virology

Cite this

Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor. / Yu, Jae Sung; Liao, Hua Xin; Gerdon, Aren E.; Huffman, Brian; Scearce, Richard M.; McAdams, Mille; Alam, S. Munir; Popernack, Paul M.; Sullivan, Nancy J.; Wright, David; Cliffel, David E.; Nabel, Gary J.; Haynes, Barton F.

In: Journal of Virological Methods, Vol. 137, No. 2, 11.2006, p. 219-228.

Research output: Contribution to journalArticle

Yu, JS, Liao, HX, Gerdon, AE, Huffman, B, Scearce, RM, McAdams, M, Alam, SM, Popernack, PM, Sullivan, NJ, Wright, D, Cliffel, DE, Nabel, GJ & Haynes, BF 2006, 'Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor', Journal of Virological Methods, vol. 137, no. 2, pp. 219-228. https://doi.org/10.1016/j.jviromet.2006.06.014
Yu, Jae Sung ; Liao, Hua Xin ; Gerdon, Aren E. ; Huffman, Brian ; Scearce, Richard M. ; McAdams, Mille ; Alam, S. Munir ; Popernack, Paul M. ; Sullivan, Nancy J. ; Wright, David ; Cliffel, David E. ; Nabel, Gary J. ; Haynes, Barton F. / Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor. In: Journal of Virological Methods. 2006 ; Vol. 137, No. 2. pp. 219-228.
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