Detection of early pregnancy factor (EPF) using the rabbit ovary and oviduct perfused in vitro

K. Sueoka, A. M. Dharmarajan, E. Michael, S. J. Atlas, E. E. Wallach

Research output: Contribution to journalArticle

Abstract

Two peaks of rabbit serum EPF activity were seen over the course of pregnancy. Rabbit ovaries with or without attached oviducts were perfused in vitro for 5 h beginning 12, 16, 24, 48, 72 and 120 h after mating. Perfused isolated ovaries did not produce EPF in vitro, but significant EPF activity was detected in the perfusate of the ovary together with oviduct. Pseudopregnant animals and those rabbits that did not ovulate exhibited no perfusate EPF activity. Perfusate EPF activity was highest at the time embryos were at the pronuclear stage and continued through the morula stage. Although the location of embryos at 72 h after mating varied between oviduct and uterus, EPF activity was maintained over the perfusion period. The results suggest that EPF release occurs within 3 h of fertilization and that the presence of the preblastocyst embryo is crucial for EPF release.

Original languageEnglish (US)
Pages (from-to)325-331
Number of pages7
JournalJournal of Reproduction and Fertility
Volume84
Issue number1
DOIs
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Physiology
  • Embryology
  • Molecular Biology
  • Obstetrics and Gynecology
  • Developmental Biology

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