Detection of dynamic substrate binding using MRI

Nirbhay N. Yadav, Xing Yang, Yuguo Li, Wenbo Li, Guanshu Liu, Peter C.M. Van Zijl

Research output: Contribution to journalArticle

Abstract

Magnetic Resonance Imaging (MRI) is rarely used for molecular binding studies and never without synthetic metallic labels. We designed an MRI approach that can specifically detect the binding of natural substrates (i.e. no chemical labels). To accomplish such detection of substrate-target interaction only, we exploit (i) the narrow resonance of aliphatic protons in free substrate for selective radio-frequency (RF) labeling and, (ii) the process of immobilisation upon binding to a solid-like target for fast magnetic transfer of this label over protons in the target backbone. This cascade of events is ultimately detected with MRI using magnetic interaction between target and water protons. We prove this principle using caffeine as a substrate in vitro and then apply it in vivo in the mouse brain. The combined effects of continuous labeling (label pumping), dynamic reversible binding, and water detection was found to enhance the detection sensitivity by about two to three orders of magnitude.

Original languageEnglish (US)
Article number10138
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

Fingerprint

Labels
Substrates
Magnetic Resonance Imaging
Spontaneous Fractures
Alcuronium
Butylene Glycols
Brain

ASJC Scopus subject areas

  • General

Cite this

Detection of dynamic substrate binding using MRI. / Yadav, Nirbhay N.; Yang, Xing; Li, Yuguo; Li, Wenbo; Liu, Guanshu; Van Zijl, Peter C.M.

In: Scientific Reports, Vol. 7, No. 1, 10138, 01.12.2017.

Research output: Contribution to journalArticle

Yadav, Nirbhay N.; Yang, Xing; Li, Yuguo; Li, Wenbo; Liu, Guanshu; Van Zijl, Peter C.M. / Detection of dynamic substrate binding using MRI.

In: Scientific Reports, Vol. 7, No. 1, 10138, 01.12.2017.

Research output: Contribution to journalArticle

@article{f64495b412ed467fa970ccc04244ffef,
title = "Detection of dynamic substrate binding using MRI",
abstract = "Magnetic Resonance Imaging (MRI) is rarely used for molecular binding studies and never without synthetic metallic labels. We designed an MRI approach that can specifically detect the binding of natural substrates (i.e. no chemical labels). To accomplish such detection of substrate-target interaction only, we exploit (i) the narrow resonance of aliphatic protons in free substrate for selective radio-frequency (RF) labeling and, (ii) the process of immobilisation upon binding to a solid-like target for fast magnetic transfer of this label over protons in the target backbone. This cascade of events is ultimately detected with MRI using magnetic interaction between target and water protons. We prove this principle using caffeine as a substrate in vitro and then apply it in vivo in the mouse brain. The combined effects of continuous labeling (label pumping), dynamic reversible binding, and water detection was found to enhance the detection sensitivity by about two to three orders of magnitude.",
author = "Yadav, {Nirbhay N.} and Xing Yang and Yuguo Li and Wenbo Li and Guanshu Liu and {Van Zijl}, {Peter C.M.}",
year = "2017",
month = "12",
doi = "10.1038/s41598-017-10545-1",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Detection of dynamic substrate binding using MRI

AU - Yadav,Nirbhay N.

AU - Yang,Xing

AU - Li,Yuguo

AU - Li,Wenbo

AU - Liu,Guanshu

AU - Van Zijl,Peter C.M.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Magnetic Resonance Imaging (MRI) is rarely used for molecular binding studies and never without synthetic metallic labels. We designed an MRI approach that can specifically detect the binding of natural substrates (i.e. no chemical labels). To accomplish such detection of substrate-target interaction only, we exploit (i) the narrow resonance of aliphatic protons in free substrate for selective radio-frequency (RF) labeling and, (ii) the process of immobilisation upon binding to a solid-like target for fast magnetic transfer of this label over protons in the target backbone. This cascade of events is ultimately detected with MRI using magnetic interaction between target and water protons. We prove this principle using caffeine as a substrate in vitro and then apply it in vivo in the mouse brain. The combined effects of continuous labeling (label pumping), dynamic reversible binding, and water detection was found to enhance the detection sensitivity by about two to three orders of magnitude.

AB - Magnetic Resonance Imaging (MRI) is rarely used for molecular binding studies and never without synthetic metallic labels. We designed an MRI approach that can specifically detect the binding of natural substrates (i.e. no chemical labels). To accomplish such detection of substrate-target interaction only, we exploit (i) the narrow resonance of aliphatic protons in free substrate for selective radio-frequency (RF) labeling and, (ii) the process of immobilisation upon binding to a solid-like target for fast magnetic transfer of this label over protons in the target backbone. This cascade of events is ultimately detected with MRI using magnetic interaction between target and water protons. We prove this principle using caffeine as a substrate in vitro and then apply it in vivo in the mouse brain. The combined effects of continuous labeling (label pumping), dynamic reversible binding, and water detection was found to enhance the detection sensitivity by about two to three orders of magnitude.

UR - http://www.scopus.com/inward/record.url?scp=85028700673&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028700673&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-10545-1

DO - 10.1038/s41598-017-10545-1

M3 - Article

VL - 7

JO - Scientific Reports

T2 - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 10138

ER -