Detection of cytomegalovirus DNA in plasma as an adjunct diagnostic for gastrointestinal tract disease in kidney and liver transplant recipients

Christine M. Durand, Kieren A. Marr, Christina A. Arnold, Lydia Tang, Daniel J. Durand, Robin K. Avery, Alexandra Valsamakis, Dionissios Neofytos

Research output: Contribution to journalArticle

Abstract

Background. Cytomegalovirus (CMV) disease is the most common infectious complication after solid organ transplantation, frequently affecting the gastrointestinal (GI) tract. There are limited data on quantitative polymerase chain reaction (qPCR) for plasma CMV DNA as an adjunct diagnostic method for GI tract disease in kidney and liver transplant recipients. Methods. We reviewed all records of adult kidney and liver transplant recipients with a GI tract biopsy and plasma CMV qPCR result within 15 days of biopsy during a 6.5-year period at our center. CMV GI tract disease was defined as histopathologic evidence of CMV on biopsy by immunohistochemistry or visualization of inclusion bodies. Results. GI tract biopsy and qPCR results were available for 81 kidney and liver transplant recipients; 20 cases of confirmed CMV GI tract disease were identified. Overall, the sensitivity of qPCR for diagnosing CMV GI tract disease was 85% (95% confidence interval [CI], 61%-96%), and the specificity was 95% (95% CI, 85%-99%). For CMV-seronegative recipients (R-) with CMV-seropositive donors (D+), the sensitivity of qPCR was 100% (95% CI, 59%-99%), and the specificity was 80% (95% CI, 30%-99%). The lowest sensitivity was observed in CMV D+/R+ cases (72.7%; 95% CI, 39%-93%). The mean plasma CMV copy number in patients with GI tract disease was 3.84 log10 (38 334 copies/mL). Conclusions. Plasma CMV qPCR had good sensitivity and excellent specificity for CMV GI tract disease in kidney and liver transplant recipients. Its sensitivity was 100% in CMV D +/R- cases but 72.7% in CMV D+/R+ cases. This variation in assay performance according to host serostatus may reflect differences in disease pathogenesis.

Original languageEnglish (US)
Pages (from-to)1550-1559
Number of pages10
JournalClinical Infectious Diseases
Volume57
Issue number11
DOIs
StatePublished - Dec 1 2013

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Keywords

  • CMV
  • Colitis
  • Kidney and liver transplant recipients
  • Viremia

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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