Detection of Clostridium botulinum type B toxin in the presence of a lethal substance interfering with toxin neutralization

Manouchehr Dezfulian, John G. Bartlett

Research output: Contribution to journalArticlepeer-review

Abstract

A low molecular weight substance that induced seizures and death in mice was present in the stool of a child with infant botulism. This interfered with mouse bioassay used for detecting Clostridium botulinum toxin. After removal of the interfering substance by a prolonged dialysis with buffered saline, in vivo neutralization of botulinal toxin was readily achieved. Alternatively, the botulinal toxin was demonstrated in stool of the infant by an enzyme-linked immunosorbent assay (ELISA), using antibodies produced in immunologically toerant rabbits.

Original languageEnglish (US)
Pages (from-to)105-112
Number of pages8
JournalDiagnostic Microbiology and Infectious Disease
Volume3
Issue number2
DOIs
StatePublished - Mar 1985
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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