TY - JOUR
T1 - Detection of Clostridium botulinum type B toxin in the presence of a lethal substance interfering with toxin neutralization
AU - Dezfulian, Manouchehr
AU - Bartlett, John G.
PY - 1985/3
Y1 - 1985/3
N2 - A low molecular weight substance that induced seizures and death in mice was present in the stool of a child with infant botulism. This interfered with mouse bioassay used for detecting Clostridium botulinum toxin. After removal of the interfering substance by a prolonged dialysis with buffered saline, in vivo neutralization of botulinal toxin was readily achieved. Alternatively, the botulinal toxin was demonstrated in stool of the infant by an enzyme-linked immunosorbent assay (ELISA), using antibodies produced in immunologically toerant rabbits.
AB - A low molecular weight substance that induced seizures and death in mice was present in the stool of a child with infant botulism. This interfered with mouse bioassay used for detecting Clostridium botulinum toxin. After removal of the interfering substance by a prolonged dialysis with buffered saline, in vivo neutralization of botulinal toxin was readily achieved. Alternatively, the botulinal toxin was demonstrated in stool of the infant by an enzyme-linked immunosorbent assay (ELISA), using antibodies produced in immunologically toerant rabbits.
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U2 - 10.1016/0732-8893(85)90018-5
DO - 10.1016/0732-8893(85)90018-5
M3 - Article
C2 - 3884243
AN - SCOPUS:0022375004
SN - 0732-8893
VL - 3
SP - 105
EP - 112
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 2
ER -