Detection of biological activity in the cerebrospinal fluid of patients with central nervous system tumors

David Tapper, Mitchell A. Cahan, Henry Brem

Research output: Contribution to journalArticlepeer-review

Abstract

Tumor markers for brain tumors are important for initial diagnosis and monitoring of treatment. We used a modification of the phagokinetic track assay, which measures the migration of cells across a coverslip that is coated with colloidal gold, to assess whether the CSF from patients with brain tumors and other non-neoplastic neurological disorders altered the migration of Balb/c 3T3 fibroblasts. We found that CSF from patients with brain tumors stimulated the migration activity at a significantly higher level than did CSF from patients without tumors (mean migration activity: 65 ±9% for CSF from 113 patients with brain tumors; 14±4% for 44 patients with non-neoplastic CNS disease; and 9 ±1.2% for 54 patients with metabolic or other disorders). Thus the ability of CSF to stimulate migration of 3T3 cells appears to be a promising approach to detecting, understanding and following the activity of brain tumors.

Original languageEnglish (US)
Pages (from-to)17-23
Number of pages7
JournalJournal of neuro-oncology
Volume19
Issue number1
DOIs
StatePublished - Feb 1 1994

Keywords

  • balb/c 3T3 cells
  • biological
  • cerebrospinal fluid
  • nervous system neoplasms
  • phagokinetic track assay
  • tumor markers

ASJC Scopus subject areas

  • Oncology
  • Neurology
  • Clinical Neurology
  • Cancer Research

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