Detection of antigen-specific T cells on p/MHC microarrays

Gokhan Deviren, Kapil Gupta, Michael E. Paulaitis, Jonathan P. Schneck

Research output: Contribution to journalArticle

Abstract

The development of high-throughput protein microarrays for rapidly determining antigen-specific T-cell receptor repertoires of diverse T-cell populations can enable comprehensive, broad-based analyses of T-cell responses. Promising applications include medical diagnostics, vaccine development, treatment of autoimmune diseases and detection of potential agents of bioterrorism. In this study, we examined the feasibility of using peptide/major histocompatibility complex (p/MHC) microarrays to selectively capture and enumerate antigen-specific T cells. Results are presented for p/MHC microarrays consisting of a dimeric MHC-immunoglobulin complex, Kb-Ig, loaded with either a cognate or non-cognate peptide for binding CD8+ T cells. We quantified the sensitivity of these Kb-Ig microarrays by measuring a lower detection limit of 0.05% antigen-specific CD8+ T cells mixed with splenocytes from C57BL/6J mouse. A fivefold increase in this lower detection limit (0.01%) was achieved using a secondary capture anti-Ig antibody to coat the microarray surface. This higher sensitivity is comparable to that obtained using standard state-of-the-art fluorescence activated cell sorting (FACS) instruments. We also found that contacting the T-cell suspension with the Kb-Ig microarrays under mild shear flow conditions produced more uniform distributions of captured T cells on the individual spots and better spot-to-spot reproducibility across the entire microarray.

Original languageEnglish (US)
Pages (from-to)32-38
Number of pages7
JournalJournal of Molecular Recognition
Volume20
Issue number1
DOIs
StatePublished - Jan 1 2007

Keywords

  • Antigens
  • Epitopes
  • High-throughput screening
  • Immune response vaccines
  • Infectious diseases
  • T cells
  • TCR
  • p/MHC microarrays

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Detection of antigen-specific T cells on p/MHC microarrays'. Together they form a unique fingerprint.

  • Cite this