Detection of antibodies against SARS-coronavirus using recombinant truncated nucleocapsid proteins by ELISA

Hyun Kyoung Lee, Byoung Hee Lee, Noton Dutta, Seung Hyeok Seok, Min Won Baek, Hui Young Lee, Dong Jae Kim, Yi Rang Na, Kyoung Jin Noh, Sung Hoon Park, Hiroaki Kariwa, Mina Nakauchi, Le Quynh Mai, Suk Jin Heo, Jae Hak Park

Research output: Contribution to journalArticle

Abstract

Severe acute respiratory syndrome (SARS) is a life-threatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1-109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis.

Original languageEnglish (US)
Pages (from-to)1717-1721
Number of pages5
JournalJournal of Microbiology and Biotechnology
Volume18
Issue number10
StatePublished - Oct 28 2008
Externally publishedYes

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Nucleocapsid Proteins
Severe Acute Respiratory Syndrome
Coronavirus
Enzyme-Linked Immunosorbent Assay
Antibodies
Serum
Western Blotting
Recombinant Proteins
Antigens
Sensitivity and Specificity

Keywords

  • ELISA
  • SARS
  • Truncated recombinant nucleocapsid proteins

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Lee, H. K., Lee, B. H., Dutta, N., Seok, S. H., Baek, M. W., Lee, H. Y., ... Park, J. H. (2008). Detection of antibodies against SARS-coronavirus using recombinant truncated nucleocapsid proteins by ELISA. Journal of Microbiology and Biotechnology, 18(10), 1717-1721.

Detection of antibodies against SARS-coronavirus using recombinant truncated nucleocapsid proteins by ELISA. / Lee, Hyun Kyoung; Lee, Byoung Hee; Dutta, Noton; Seok, Seung Hyeok; Baek, Min Won; Lee, Hui Young; Kim, Dong Jae; Na, Yi Rang; Noh, Kyoung Jin; Park, Sung Hoon; Kariwa, Hiroaki; Nakauchi, Mina; Mai, Le Quynh; Heo, Suk Jin; Park, Jae Hak.

In: Journal of Microbiology and Biotechnology, Vol. 18, No. 10, 28.10.2008, p. 1717-1721.

Research output: Contribution to journalArticle

Lee, HK, Lee, BH, Dutta, N, Seok, SH, Baek, MW, Lee, HY, Kim, DJ, Na, YR, Noh, KJ, Park, SH, Kariwa, H, Nakauchi, M, Mai, LQ, Heo, SJ & Park, JH 2008, 'Detection of antibodies against SARS-coronavirus using recombinant truncated nucleocapsid proteins by ELISA', Journal of Microbiology and Biotechnology, vol. 18, no. 10, pp. 1717-1721.
Lee, Hyun Kyoung ; Lee, Byoung Hee ; Dutta, Noton ; Seok, Seung Hyeok ; Baek, Min Won ; Lee, Hui Young ; Kim, Dong Jae ; Na, Yi Rang ; Noh, Kyoung Jin ; Park, Sung Hoon ; Kariwa, Hiroaki ; Nakauchi, Mina ; Mai, Le Quynh ; Heo, Suk Jin ; Park, Jae Hak. / Detection of antibodies against SARS-coronavirus using recombinant truncated nucleocapsid proteins by ELISA. In: Journal of Microbiology and Biotechnology. 2008 ; Vol. 18, No. 10. pp. 1717-1721.
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