Detection of Anaplasma phagocytophilum DNA in Ixodes ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, mainland Portugal

Ana Sofia Santos, Maria Margarida Santos-Silva, Victor Carlos Almeida, Fátima Bacellar, John Stephen Dumler

Research output: Contribution to journalArticle

Abstract

A total of 278 Ixodes ticks, collected from Madeira Island and Setubal District, mainland Portugal, were examined by polymerase chain reaction (PCR) for the presence of Anaplasma phagocytophilum. Six (4%) of 142 Ixodes ricinus nymphs collected in Madeira Island and 1 nymph and 1 male (2%) of 93 I. ventalloi collected in Setúbal District tested positive for A. phagocytophilum msp2 genes or rrs. Infection was not detected among 43 I. ricinus on mainland Portugal. All PCR products were confirmed by nucleotide sequencing to be identical or to be most closely related to A. phagocytophilum. To our knowledge, this is the first evidence of A. phagocytophilum in ticks from Setúbal District, mainland Portugal, and the first documentation of Anaplasma infection in I. ventalloi. Moreover, these findings confirm the persistence of A. phagocytophilum in Madeira Island's I. ricinus.

Original languageEnglish (US)
Pages (from-to)1643-1648
Number of pages6
JournalEmerging Infectious Diseases
Volume10
Issue number9
StatePublished - Sep 2004

Fingerprint

Anaplasma phagocytophilum
Ixodidae
Ixodes
Portugal
Ticks
DNA
Nymph
Anaplasma
Polymerase Chain Reaction
Infection
Documentation
Nucleotides
Genes

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Santos, A. S., Santos-Silva, M. M., Almeida, V. C., Bacellar, F., & Dumler, J. S. (2004). Detection of Anaplasma phagocytophilum DNA in Ixodes ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, mainland Portugal. Emerging Infectious Diseases, 10(9), 1643-1648.

Detection of Anaplasma phagocytophilum DNA in Ixodes ticks (Acari : Ixodidae) from Madeira Island and Setúbal District, mainland Portugal. / Santos, Ana Sofia; Santos-Silva, Maria Margarida; Almeida, Victor Carlos; Bacellar, Fátima; Dumler, John Stephen.

In: Emerging Infectious Diseases, Vol. 10, No. 9, 09.2004, p. 1643-1648.

Research output: Contribution to journalArticle

Santos, AS, Santos-Silva, MM, Almeida, VC, Bacellar, F & Dumler, JS 2004, 'Detection of Anaplasma phagocytophilum DNA in Ixodes ticks (Acari: Ixodidae) from Madeira Island and Setúbal District, mainland Portugal', Emerging Infectious Diseases, vol. 10, no. 9, pp. 1643-1648.
Santos, Ana Sofia ; Santos-Silva, Maria Margarida ; Almeida, Victor Carlos ; Bacellar, Fátima ; Dumler, John Stephen. / Detection of Anaplasma phagocytophilum DNA in Ixodes ticks (Acari : Ixodidae) from Madeira Island and Setúbal District, mainland Portugal. In: Emerging Infectious Diseases. 2004 ; Vol. 10, No. 9. pp. 1643-1648.
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