Detection of an adherence factor of enteropathogenic escherichia coli with a dna probe

James P. Nataro; Mary M. Baldini; James B. Kaper; Robert E. Black; Nora Bravo; Myron M. Levine

doi:10.1093/infdis/152.3.560

Detection of an adherence factor of enteropathogenic escherichia coli with a dna probe

James P. Nataro, Mary M. Baldini, James B. Kaper, Robert E. Black, Nora Bravo, Myron M. Levine

Research output: Contribution to journal › Article › peer-review

261 Scopus citations

Abstract

A DNA probe to detect genes conferring localized adherence of enteropathogenic Escherichia coli (EPEC) to Hep-2 cells was evaluated by using E. coliisolates from the stools of Peruvian infants with and without diarrhea. The probe was both sensitive and specific and revealed that Hep-2 adherence (because of the EPEC adherence factor [EAF]) was more frequent in some O serogroups of EPEC than in others. Those serogroups in which EAF is almost always found have been designated class I EPEC; serogroups in which EAF is rarely found have been designated class II. Both class I (EAF-positive) and class II EPEC are associated with diarrheal disease.

Original language	English (US)
Pages (from-to)	560-565
Number of pages	6
Journal	Journal of Infectious Diseases
Volume	152
Issue number	3
DOIs	https://doi.org/10.1093/infdis/152.3.560
State	Published - Sep 1 1985
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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10.1093/infdis/152.3.560

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title = "Detection of an adherence factor of enteropathogenic escherichia coli with a dna probe",

abstract = "A DNA probe to detect genes conferring localized adherence of enteropathogenic Escherichia coli (EPEC) to Hep-2 cells was evaluated by using E. coliisolates from the stools of Peruvian infants with and without diarrhea. The probe was both sensitive and specific and revealed that Hep-2 adherence (because of the EPEC adherence factor [EAF]) was more frequent in some O serogroups of EPEC than in others. Those serogroups in which EAF is almost always found have been designated class I EPEC; serogroups in which EAF is rarely found have been designated class II. Both class I (EAF-positive) and class II EPEC are associated with diarrheal disease.",

author = "Nataro, {James P.} and Baldini, {Mary M.} and Kaper, {James B.} and Black, {Robert E.} and Nora Bravo and Levine, {Myron M.}",

note = "Funding Information: This work was supported by contract NOI-AI-12666 from the National Institute of Allergy and Infectious Diseases and a grant from the World Health Organization.",

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T1 - Detection of an adherence factor of enteropathogenic escherichia coli with a dna probe

AU - Nataro, James P.

AU - Baldini, Mary M.

AU - Kaper, James B.

AU - Black, Robert E.

AU - Bravo, Nora

AU - Levine, Myron M.

N1 - Funding Information: This work was supported by contract NOI-AI-12666 from the National Institute of Allergy and Infectious Diseases and a grant from the World Health Organization.

PY - 1985/9/1

Y1 - 1985/9/1

N2 - A DNA probe to detect genes conferring localized adherence of enteropathogenic Escherichia coli (EPEC) to Hep-2 cells was evaluated by using E. coliisolates from the stools of Peruvian infants with and without diarrhea. The probe was both sensitive and specific and revealed that Hep-2 adherence (because of the EPEC adherence factor [EAF]) was more frequent in some O serogroups of EPEC than in others. Those serogroups in which EAF is almost always found have been designated class I EPEC; serogroups in which EAF is rarely found have been designated class II. Both class I (EAF-positive) and class II EPEC are associated with diarrheal disease.

AB - A DNA probe to detect genes conferring localized adherence of enteropathogenic Escherichia coli (EPEC) to Hep-2 cells was evaluated by using E. coliisolates from the stools of Peruvian infants with and without diarrhea. The probe was both sensitive and specific and revealed that Hep-2 adherence (because of the EPEC adherence factor [EAF]) was more frequent in some O serogroups of EPEC than in others. Those serogroups in which EAF is almost always found have been designated class I EPEC; serogroups in which EAF is rarely found have been designated class II. Both class I (EAF-positive) and class II EPEC are associated with diarrheal disease.

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Detection of an adherence factor of enteropathogenic escherichia coli with a dna probe

Abstract

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