Detection of an adherence factor of enteropathogenic escherichia coli with a dna probe

James P. Nataro, Mary M. Baldini, James B. Kaper, Robert E. Black, Nora Bravo, Myron M. Levine

Research output: Contribution to journalArticlepeer-review

Abstract

A DNA probe to detect genes conferring localized adherence of enteropathogenic Escherichia coli (EPEC) to Hep-2 cells was evaluated by using E. coliisolates from the stools of Peruvian infants with and without diarrhea. The probe was both sensitive and specific and revealed that Hep-2 adherence (because of the EPEC adherence factor [EAF]) was more frequent in some O serogroups of EPEC than in others. Those serogroups in which EAF is almost always found have been designated class I EPEC; serogroups in which EAF is rarely found have been designated class II. Both class I (EAF-positive) and class II EPEC are associated with diarrheal disease.

Original languageEnglish (US)
Pages (from-to)560-565
Number of pages6
JournalJournal of Infectious Diseases
Volume152
Issue number3
DOIs
StatePublished - Sep 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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