Detection of a wide variety of human and veterinary fluoroquinolone antibiotics in municipal wastewater and wastewater-impacted surface water

Ke He, Ana Dulce Soares, Hollie Adejumo, Melissa McDiarmid, Katherine Squibb, Lee Blaney

Research output: Contribution to journalArticle

Abstract

As annual sales of antibiotics continue to rise, the mass of these specially-designed compounds entering municipal wastewater treatment systems has also increased. Of primary concern here is that antibiotics can inhibit growth of specific microorganisms in biological processes of wastewater treatment plants (WWTPs) or in downstream ecosystems. Growth inhibition studies with Escherichia coli demonstrated that solutions containing 1-10. μg/L of fluoroquinolones can inhibit microbial growth. Wastewater samples were collected on a monthly basis from various treatment stages of a 30 million gallon per day WWTP in Maryland, USA. Samples were analyzed for the presence of 11 fluoroquinolone antibiotics. At least one fluoroquinolone was detected in every sample. Ofloxacin and ciprofloxacin exhibited detection frequencies of 100% and 98%, respectively, across all sampling sites. Concentrations of fluoroquinolones in raw wastewater were as high as 1900. ng/L for ciprofloxacin and 600. ng/L for ofloxacin. Difloxacin, enrofloxacin, fleroxacin, moxifloxacin, norfloxacin, and orbifloxacin were also detected at appreciable concentrations of 9-170. ng/L. The total mass concentration of fluoroquinolones in raw wastewater was in the range that inhibited E. coli growth, suggesting that concerns over antibiotic presence in wastewater and wastewater-impacted surface water are valid. The average removal efficiency of fluoroquinolones during wastewater treatment was approximately 65%; furthermore, the removal efficiency for fluoroquinolones was found to be negatively correlated to biochemical oxygen demand removal and positively correlated to phosphorus removal.

Original languageEnglish (US)
Pages (from-to)136-143
Number of pages8
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume106
DOIs
StatePublished - Mar 5 2015
Externally publishedYes

Fingerprint

Fluoroquinolones
Waste Water
Surface waters
Wastewater
Anti-Bacterial Agents
Water
Wastewater treatment
Ofloxacin
Ciprofloxacin
difloxacin
Escherichia coli
Growth
Fleroxacin
Norfloxacin
Biochemical oxygen demand
Biological Phenomena
Microorganisms
Ecosystems
Phosphorus
Sales

Keywords

  • Antibiotics
  • Ciprofloxacin
  • Fluoroquinolones
  • Ofloxacin
  • Wastewater

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry

Cite this

Detection of a wide variety of human and veterinary fluoroquinolone antibiotics in municipal wastewater and wastewater-impacted surface water. / He, Ke; Soares, Ana Dulce; Adejumo, Hollie; McDiarmid, Melissa; Squibb, Katherine; Blaney, Lee.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 106, 05.03.2015, p. 136-143.

Research output: Contribution to journalArticle

He, Ke ; Soares, Ana Dulce ; Adejumo, Hollie ; McDiarmid, Melissa ; Squibb, Katherine ; Blaney, Lee. / Detection of a wide variety of human and veterinary fluoroquinolone antibiotics in municipal wastewater and wastewater-impacted surface water. In: Journal of Pharmaceutical and Biomedical Analysis. 2015 ; Vol. 106. pp. 136-143.
@article{c99b891134554a39a9277f6d81fcd94a,
title = "Detection of a wide variety of human and veterinary fluoroquinolone antibiotics in municipal wastewater and wastewater-impacted surface water",
abstract = "As annual sales of antibiotics continue to rise, the mass of these specially-designed compounds entering municipal wastewater treatment systems has also increased. Of primary concern here is that antibiotics can inhibit growth of specific microorganisms in biological processes of wastewater treatment plants (WWTPs) or in downstream ecosystems. Growth inhibition studies with Escherichia coli demonstrated that solutions containing 1-10. μg/L of fluoroquinolones can inhibit microbial growth. Wastewater samples were collected on a monthly basis from various treatment stages of a 30 million gallon per day WWTP in Maryland, USA. Samples were analyzed for the presence of 11 fluoroquinolone antibiotics. At least one fluoroquinolone was detected in every sample. Ofloxacin and ciprofloxacin exhibited detection frequencies of 100{\%} and 98{\%}, respectively, across all sampling sites. Concentrations of fluoroquinolones in raw wastewater were as high as 1900. ng/L for ciprofloxacin and 600. ng/L for ofloxacin. Difloxacin, enrofloxacin, fleroxacin, moxifloxacin, norfloxacin, and orbifloxacin were also detected at appreciable concentrations of 9-170. ng/L. The total mass concentration of fluoroquinolones in raw wastewater was in the range that inhibited E. coli growth, suggesting that concerns over antibiotic presence in wastewater and wastewater-impacted surface water are valid. The average removal efficiency of fluoroquinolones during wastewater treatment was approximately 65{\%}; furthermore, the removal efficiency for fluoroquinolones was found to be negatively correlated to biochemical oxygen demand removal and positively correlated to phosphorus removal.",
keywords = "Antibiotics, Ciprofloxacin, Fluoroquinolones, Ofloxacin, Wastewater",
author = "Ke He and Soares, {Ana Dulce} and Hollie Adejumo and Melissa McDiarmid and Katherine Squibb and Lee Blaney",
year = "2015",
month = "3",
day = "5",
doi = "10.1016/j.jpba.2014.11.020",
language = "English (US)",
volume = "106",
pages = "136--143",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",

}

TY - JOUR

T1 - Detection of a wide variety of human and veterinary fluoroquinolone antibiotics in municipal wastewater and wastewater-impacted surface water

AU - He, Ke

AU - Soares, Ana Dulce

AU - Adejumo, Hollie

AU - McDiarmid, Melissa

AU - Squibb, Katherine

AU - Blaney, Lee

PY - 2015/3/5

Y1 - 2015/3/5

N2 - As annual sales of antibiotics continue to rise, the mass of these specially-designed compounds entering municipal wastewater treatment systems has also increased. Of primary concern here is that antibiotics can inhibit growth of specific microorganisms in biological processes of wastewater treatment plants (WWTPs) or in downstream ecosystems. Growth inhibition studies with Escherichia coli demonstrated that solutions containing 1-10. μg/L of fluoroquinolones can inhibit microbial growth. Wastewater samples were collected on a monthly basis from various treatment stages of a 30 million gallon per day WWTP in Maryland, USA. Samples were analyzed for the presence of 11 fluoroquinolone antibiotics. At least one fluoroquinolone was detected in every sample. Ofloxacin and ciprofloxacin exhibited detection frequencies of 100% and 98%, respectively, across all sampling sites. Concentrations of fluoroquinolones in raw wastewater were as high as 1900. ng/L for ciprofloxacin and 600. ng/L for ofloxacin. Difloxacin, enrofloxacin, fleroxacin, moxifloxacin, norfloxacin, and orbifloxacin were also detected at appreciable concentrations of 9-170. ng/L. The total mass concentration of fluoroquinolones in raw wastewater was in the range that inhibited E. coli growth, suggesting that concerns over antibiotic presence in wastewater and wastewater-impacted surface water are valid. The average removal efficiency of fluoroquinolones during wastewater treatment was approximately 65%; furthermore, the removal efficiency for fluoroquinolones was found to be negatively correlated to biochemical oxygen demand removal and positively correlated to phosphorus removal.

AB - As annual sales of antibiotics continue to rise, the mass of these specially-designed compounds entering municipal wastewater treatment systems has also increased. Of primary concern here is that antibiotics can inhibit growth of specific microorganisms in biological processes of wastewater treatment plants (WWTPs) or in downstream ecosystems. Growth inhibition studies with Escherichia coli demonstrated that solutions containing 1-10. μg/L of fluoroquinolones can inhibit microbial growth. Wastewater samples were collected on a monthly basis from various treatment stages of a 30 million gallon per day WWTP in Maryland, USA. Samples were analyzed for the presence of 11 fluoroquinolone antibiotics. At least one fluoroquinolone was detected in every sample. Ofloxacin and ciprofloxacin exhibited detection frequencies of 100% and 98%, respectively, across all sampling sites. Concentrations of fluoroquinolones in raw wastewater were as high as 1900. ng/L for ciprofloxacin and 600. ng/L for ofloxacin. Difloxacin, enrofloxacin, fleroxacin, moxifloxacin, norfloxacin, and orbifloxacin were also detected at appreciable concentrations of 9-170. ng/L. The total mass concentration of fluoroquinolones in raw wastewater was in the range that inhibited E. coli growth, suggesting that concerns over antibiotic presence in wastewater and wastewater-impacted surface water are valid. The average removal efficiency of fluoroquinolones during wastewater treatment was approximately 65%; furthermore, the removal efficiency for fluoroquinolones was found to be negatively correlated to biochemical oxygen demand removal and positively correlated to phosphorus removal.

KW - Antibiotics

KW - Ciprofloxacin

KW - Fluoroquinolones

KW - Ofloxacin

KW - Wastewater

UR - http://www.scopus.com/inward/record.url?scp=84923591839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923591839&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2014.11.020

DO - 10.1016/j.jpba.2014.11.020

M3 - Article

C2 - 25483174

AN - SCOPUS:84923591839

VL - 106

SP - 136

EP - 143

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

ER -