Detection and quantitation of bryostatin 1 and 2 in bugula neritina by combined high-peformance liquid chromatography and3h-phorbol dibutyrate displacement

Daniel E. Schaufelberger, Ada Belinda Alvarado, Pamela Andrews, John A. Beutler

Research output: Contribution to journalArticlepeer-review

Abstract

An HPLC method has been developed to detect and quantitate bryostatins 1 and 2 from crude extracts of B. neritina. This, coupled with a3H-phorbol dibutyrate binding assay and photodiode array-acquired uv spectra makes possible the evaluation of crude extracts in a rapid, sensitive and specific manner.

Original languageEnglish (US)
Pages (from-to)597-598
Number of pages2
JournalJournal of Liquid Chromatography
Volume13
Issue number3
DOIs
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine

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