Detection and localization of a chloroplast-encoded HU-like protein that organizes chloroplast nucleoids

Tamaki Kobayashi, Manabu Takahara, Shin Ya Miyagishima, Haruko Kuroiwa, Narie Sasaki, Niji Ohta, Motomichi Matsuzaki, Tsuneyoshi Kuroiwa

Research output: Contribution to journalArticle

Abstract

Chloroplast DNA (cpDNA) is packed into discrete structures called chloroplast nucleoids (cp-nucleoids). The structure of cpDNA is thought to be important for its maintenance and regulation. In bacteria and mitochondria, histone-like proteins (such as HU and Abf2, respectively) are abundant and play important roles in DNA organization. However, a primary structural protein has yet to be found in cp-nucleoids. Here, we identified an abundant DNA binding protein from isolated cp-nucleoids of the primitive red alga Cyanidioschyzon merolae. The purified protein had sequence homology with the bacterial histone-like protein HU, and it complemented HU-lacking Escherichia coli mutants. The protein, called HC (histone-like protein of chloroplast), was encoded by a single gene (CmhupA) in the C. merolae chloroplast genome. Using immunofluorescence and immunoelectron microscopy, we demonstrated that HC was distributed uniformly throughout the entire cp-nucleoid. The protein was expressed constitutively throughout the cell and the chloroplast division cycle, and it was able to condense DNA. These results indicate that HC, a bacteria-derived histone-like protein, primarily organizes cpDNA into the nucleoid.

Original languageEnglish (US)
Pages (from-to)1579-1589
Number of pages11
JournalPlant Cell
Volume14
Issue number7
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Chloroplast Proteins
Chloroplasts
Chloroplast DNA
chloroplasts
Histones
histones
chloroplast DNA
Proteins
proteins
Chloroplast Genome
Bacteria
Rhodophyta
Amino Acid Sequence Homology
Genes
Immunoelectron Microscopy
DNA
DNA-Binding Proteins
Mitochondria
DNA-binding proteins
Fluorescence Microscopy

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Kobayashi, T., Takahara, M., Miyagishima, S. Y., Kuroiwa, H., Sasaki, N., Ohta, N., ... Kuroiwa, T. (2002). Detection and localization of a chloroplast-encoded HU-like protein that organizes chloroplast nucleoids. Plant Cell, 14(7), 1579-1589. https://doi.org/10.1105/tpc.002717

Detection and localization of a chloroplast-encoded HU-like protein that organizes chloroplast nucleoids. / Kobayashi, Tamaki; Takahara, Manabu; Miyagishima, Shin Ya; Kuroiwa, Haruko; Sasaki, Narie; Ohta, Niji; Matsuzaki, Motomichi; Kuroiwa, Tsuneyoshi.

In: Plant Cell, Vol. 14, No. 7, 2002, p. 1579-1589.

Research output: Contribution to journalArticle

Kobayashi, T, Takahara, M, Miyagishima, SY, Kuroiwa, H, Sasaki, N, Ohta, N, Matsuzaki, M & Kuroiwa, T 2002, 'Detection and localization of a chloroplast-encoded HU-like protein that organizes chloroplast nucleoids', Plant Cell, vol. 14, no. 7, pp. 1579-1589. https://doi.org/10.1105/tpc.002717
Kobayashi, Tamaki ; Takahara, Manabu ; Miyagishima, Shin Ya ; Kuroiwa, Haruko ; Sasaki, Narie ; Ohta, Niji ; Matsuzaki, Motomichi ; Kuroiwa, Tsuneyoshi. / Detection and localization of a chloroplast-encoded HU-like protein that organizes chloroplast nucleoids. In: Plant Cell. 2002 ; Vol. 14, No. 7. pp. 1579-1589.
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