Detection and comparison of epidermal growth factor receptor mutations in cells and fluid of malignant pleural effusion in non-small cell lung cancer

Xiaozhu Zhang, Yi Zhao, Miao Wang, Wee See Yap, Alex Yuang chi Chang

Research output: Contribution to journalArticlepeer-review

Abstract

Cells or cell-free fluid of malignant pleural effusion could be important clinical specimen for epidermal growth factor receptor (EGFR) mutation screening in advanced non-small cell lung cancer (NSCLC) patients. However, their usefulness in mutation detection has not been well compared. In this study we recruited 26 East Asian NSCLC patients with malignant pleural effusion, determined the mutation status of EGFR in both cells and matched cell-free fluid with the use of sequencing and mutant-enriched PCR. After comparing the mutation spectrums, we found both the cells and cell-free pleural fluid may be feasible clinical specimen for EGFR mutation detection in unresectable NSCLC given sensitive genotyping assays employed. Direct sequencing could miss a significant portion of mutations in these heterogeneous specimens. More sensitive methods, such as mutant-enriched PCR and gene scan, could provide more reliable mutational information.

Original languageEnglish (US)
Pages (from-to)175-182
Number of pages8
JournalLung Cancer
Volume60
Issue number2
DOIs
StatePublished - May 2008
Externally publishedYes

Keywords

  • EGFR mutation
  • Malignant pleural effusion
  • Mutant-enriched PCR
  • NSCLC
  • Sequencing

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine
  • Cancer Research

Fingerprint Dive into the research topics of 'Detection and comparison of epidermal growth factor receptor mutations in cells and fluid of malignant pleural effusion in non-small cell lung cancer'. Together they form a unique fingerprint.

Cite this